Effects of Early-Acting Cytokines on the Formation of NK Cell Colonies
| Cytokines . | No. of Colonies . | |||
|---|---|---|---|---|
| . | Mixed-NK . | Pure-NK . | Pre-B . | Myeloid . |
| IL-2, IL-7, SF | 0 ± 1 | 1 ± 1 | 5 ± 1 | 8 ± 2 |
| IL-11, IL-2, IL-7, SF | 9 ± 1 | 1 ± 1 | 2 ± 1 | 11 ± 3 |
| IL-6, IL-2, IL-7, SF | 5 ± 1 | 0 ± 1 | 1 ± 1 | 8 ± 2 |
| G-CSF, IL-2, IL-7, SF | 6 ± 1 | 1 ± 1 | 3 ± 1 | 7 ± 2 |
| IL-12, IL-2, IL-7, SF | 3 ± 1 | 0 | 4 ± 1 | 8 ± 1 |
| Cytokines . | No. of Colonies . | |||
|---|---|---|---|---|
| . | Mixed-NK . | Pure-NK . | Pre-B . | Myeloid . |
| IL-2, IL-7, SF | 0 ± 1 | 1 ± 1 | 5 ± 1 | 8 ± 2 |
| IL-11, IL-2, IL-7, SF | 9 ± 1 | 1 ± 1 | 2 ± 1 | 11 ± 3 |
| IL-6, IL-2, IL-7, SF | 5 ± 1 | 0 ± 1 | 1 ± 1 | 8 ± 2 |
| G-CSF, IL-2, IL-7, SF | 6 ± 1 | 1 ± 1 | 3 ± 1 | 7 ± 2 |
| IL-12, IL-2, IL-7, SF | 3 ± 1 | 0 | 4 ± 1 | 8 ± 1 |
Sca-1+c-kit+ cells were purified from fetal liver. A total of 50 cells was plated in methylcellulose media in the presence of designated cytokines. Colonies were scored after 18 days of incubation. Data represent mean ± SD of quadruplicate cultures.