Table 1. Primers and PCR Conditions PCR... | American Society of Hematology

Table 1.

Primers and PCR Conditions

PCR Product	Genbank Accession No.	Primer Sequence (5′ ; 3′ )	Cycles	Anneal Temperature (°C)
G3PDH	M33197	GCCACATCGCTAAGACACCATGGG;	31	59
	M32599	CCTGGTGACCAGGCGCCCAAT
Mig	X72755	TTCCTCTTGGGCATCATCTTGCTG;	31	59
	S60728	GGTCTTTCAAGGATTGTAGGTGGA
IP-10	X02530	GGAACCTCCAGTCTCAGCACC;	31	59
	M17752	GCGTACGGTTCTAGAGAGAGGTAC
IFN-γ	V00536	GGACCCATATGTAAAAGAAGCAGA;	35	57
	AO4665	TGTCACTCTCCTCTTTCCAATTCT
TNF-α	M10988	AGAGGGAAGAGTCCCCCAGGA;	35	59
		TCAGCTTGAGGGTTTGCTACAA
MIP-1α	M25315	CTCTGCACCATGGCTCTCTGCAAC;	35	62
		TGTGGAATCTGCCGGGAGGTGTAG
MIP-1β	M25316	CTCCTCATGCTAGTAGCTGCCTTC;	35	62
		GGTGTAAGAAAAGCAGCAGGCGGT

PCR Product	Genbank Accession No.	Primer Sequence (5′ ; 3′ )	Cycles	Anneal Temperature (°C)
G3PDH	M33197	GCCACATCGCTAAGACACCATGGG;	31	59
	M32599	CCTGGTGACCAGGCGCCCAAT
Mig	X72755	TTCCTCTTGGGCATCATCTTGCTG;	31	59
	S60728	GGTCTTTCAAGGATTGTAGGTGGA
IP-10	X02530	GGAACCTCCAGTCTCAGCACC;	31	59
	M17752	GCGTACGGTTCTAGAGAGAGGTAC
IFN-γ	V00536	GGACCCATATGTAAAAGAAGCAGA;	35	57
	AO4665	TGTCACTCTCCTCTTTCCAATTCT
TNF-α	M10988	AGAGGGAAGAGTCCCCCAGGA;	35	59
		TCAGCTTGAGGGTTTGCTACAA
MIP-1α	M25315	CTCTGCACCATGGCTCTCTGCAAC;	35	62
		TGTGGAATCTGCCGGGAGGTGTAG
MIP-1β	M25316	CTCCTCATGCTAGTAGCTGCCTTC;	35	62
		GGTGTAAGAAAAGCAGCAGGCGGT

Abbreviations: PCR, polymerase chain reaction; IP-10, interferon-γ–inducible protein-10; IFN-γ; interferon-γ; TNF-α, tumor necrosis factor-α; MIP-1, macrophage inflammatory protein-1.