Table 1. Effects of Estrogen on Colony... | American Society of Hematology

Table 1.

Effects of Estrogen on Colony Formation by Murine Bone Marrow Cells Transduced With Selectable Amplifier Genes and Detection of Transgenes by PCR Analysis

Transgene	None		rhG-CSF (10 ng/mL)		β-Estradiol (10⁻⁷ mmol/L)
	No. of Colonies^*	PCR^†	No. of Colonies^*	PCR^†	No. of Colonies^*	PCR^†
Experiment 1
GCRER	6	6/6	77	12/12	27	11/11
None	ND	ND	15	0/7	0	ND
Experiment 2
GCRER	4 ± 2	ND	163 ± 24	ND	19 ± 5	ND
Δ(5-195)GCRER	32 ± 26	ND	205 ± 37	ND	121 ± 16	ND
LacZ	0	ND	122 ± 26	ND	0	ND
None	0	ND	144 ± 8	ND	0	ND

Transgene	None		rhG-CSF (10 ng/mL)		β-Estradiol (10⁻⁷ mmol/L)
	No. of Colonies^*	PCR^†	No. of Colonies^*	PCR^†	No. of Colonies^*	PCR^†
Experiment 1
GCRER	6	6/6	77	12/12	27	11/11
None	ND	ND	15	0/7	0	ND
Experiment 2
GCRER	4 ± 2	ND	163 ± 24	ND	19 ± 5	ND
Δ(5-195)GCRER	32 ± 26	ND	205 ± 37	ND	121 ± 16	ND
LacZ	0	ND	122 ± 26	ND	0	ND
None	0	ND	144 ± 8	ND	0	ND

Abbreviation: ND, not done.

Each value in experiment 2 is a mean of triplicate cultures (mean ± SD).

†

No. of colonies containing the transgene/no. of colonies examined.

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