Induction of Adhesion Molecules on HUVAC by HIV-tat
| Treatment . | Concentration (ng/mL) . | Mean Channel Fluorescence Intensity . | ||
|---|---|---|---|---|
| . | . | ICAM-1 . | VCAM-1 . | ELAM-1 . |
| Experiment no. 1 | ||||
| None | 0 | 14 | 13 | 14 |
| HIV-tat | 20 | 37 | 33 | 34 |
| HIV-tat + CHX | 18 | 19 | 19 | |
| HIV-tat | 20 | 64 | 28 | 47 |
| TNF | 0.2 | 80 | 31 | 62 |
| HIV-tat + TNF | 97 | 52 | 82 | |
| Experiment no. 2 | ||||
| None | 0 | 18 | 18 | 18 |
| HIV-tat | 20 | 41 | 37 | 47 |
| HIV-tat + CHX | 18 | 18 | 18 | |
| HIV-tat | 20 | 48 | 23 | 39 |
| TNF | 0.2 | 62 | 28 | 52 |
| HIV-tat + TNF | 82 | 45 | 72 | |
| Treatment . | Concentration (ng/mL) . | Mean Channel Fluorescence Intensity . | ||
|---|---|---|---|---|
| . | . | ICAM-1 . | VCAM-1 . | ELAM-1 . |
| Experiment no. 1 | ||||
| None | 0 | 14 | 13 | 14 |
| HIV-tat | 20 | 37 | 33 | 34 |
| HIV-tat + CHX | 18 | 19 | 19 | |
| HIV-tat | 20 | 64 | 28 | 47 |
| TNF | 0.2 | 80 | 31 | 62 |
| HIV-tat + TNF | 97 | 52 | 82 | |
| Experiment no. 2 | ||||
| None | 0 | 18 | 18 | 18 |
| HIV-tat | 20 | 41 | 37 | 47 |
| HIV-tat + CHX | 18 | 18 | 18 | |
| HIV-tat | 20 | 48 | 23 | 39 |
| TNF | 0.2 | 62 | 28 | 52 |
| HIV-tat + TNF | 82 | 45 | 72 | |
Human umbilical vein EC (passage 3) were treated with HIV-tat alone or in the presence of cycloheximide (CHX) or TNF for 6 hours at 37°C. At the end of incubation, cells were harvested and examined for the expression of adhesion molecules by flow cytometry. The data shown are derived from two separate experiments.