The Number of Cells (Based on a Total of 10,000 Cells) of Each Type in Each Density Fraction
Experiment No. . | RBCs . | F+ . | T+ (after permeabilization) . | T+F+ . | T+F− . | |||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
. | I . | II . | III . | Total . | I . | II . | III . | Total . | I . | II . | III . | Total . | I . | II . | III . | Total . | I . | II . | III . | Total . |
1 | 400 | 6,000 | 3,600 | 10,000 | 85 | 3,288 | 2,326 | 5,699 | 218 | 636 | 18 | 872 | 34 | 72 | 0 | 106 | 184 | 564 | 18 | 766 |
2 | 2,400 | 5,300 | 2,300 | 10,000 | 228 | 1,454 | 359 | 2,041 | 756 | 150 | 5 | 911 | 41 | 7 | 0 | 48 | 715 | 143 | 5 | 863 |
3 | 700 | 1,500 | 7,800 | 10,000 | 189 | 407 | 2,156 | 2,752 | 53 | 137 | 152 | 342 | 5 | 14 | 19 | 38 | 48 | 123 | 133 | 304 |
4 | 400 | 3,000 | 6,600 | 10,000 | 155 | 1,050 | 1,842 | 3,047 | 64 | 375 | 198 | 637 | 5 | 63 | 20 | 88 | 59 | 312 | 178 | 549 |
5 | 750 | 4,800 | 4,450 | 10,000 | 333 | 3,034 | 2,416 | 5,783 | 124 | 149 | 18 | 291 | 41 | 58 | 0 | 99 | 83 | 91 | 18 | 192 |
6 | 140 | 6,430 | 3,420 | 10,000 | 31 | 2,793 | 1485 | 4,309 | 6 | 118 | 16 | 140 | 1 | 28 | 4 | 33 | 5 | 90 | 12 | 107 |
7 | 480 | 4,760 | 4,760 | 10,000 | 48 | 493 | 615 | 1,156 | 22 | 165 | 42 | 229 | 0 | 17 | 1 | 18 | 22 | 148 | 41 | 211 |
Average | 753 | 4,541 | 4,705 | 10,000 | 153 | 1,788 | 1,600 | 3,541 | 178 | 247 | 64 | 489 | 18 | 37 | 6 | 61 | 159 | 210 | 58 | 427 |
C1* | 3,840 | 5,361 | 798 | 10,000 | 342 | 64 | 7 | 413 | ||||||||||||
C2† | 8,000 | 1,600 | 400 | 10,000 | 128 | 10 | 5 | 143 | ||||||||||||
C3‡ | 1,440 | 6,060 | 2,500 | 10,000 | 190 | 120 | 20 | 330 | ||||||||||||
Average | 4,427 | 4,340 | 1,233 | 10,000 | 220 | 65 | 11 | 295 |
Experiment No. . | RBCs . | F+ . | T+ (after permeabilization) . | T+F+ . | T+F− . | |||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
. | I . | II . | III . | Total . | I . | II . | III . | Total . | I . | II . | III . | Total . | I . | II . | III . | Total . | I . | II . | III . | Total . |
1 | 400 | 6,000 | 3,600 | 10,000 | 85 | 3,288 | 2,326 | 5,699 | 218 | 636 | 18 | 872 | 34 | 72 | 0 | 106 | 184 | 564 | 18 | 766 |
2 | 2,400 | 5,300 | 2,300 | 10,000 | 228 | 1,454 | 359 | 2,041 | 756 | 150 | 5 | 911 | 41 | 7 | 0 | 48 | 715 | 143 | 5 | 863 |
3 | 700 | 1,500 | 7,800 | 10,000 | 189 | 407 | 2,156 | 2,752 | 53 | 137 | 152 | 342 | 5 | 14 | 19 | 38 | 48 | 123 | 133 | 304 |
4 | 400 | 3,000 | 6,600 | 10,000 | 155 | 1,050 | 1,842 | 3,047 | 64 | 375 | 198 | 637 | 5 | 63 | 20 | 88 | 59 | 312 | 178 | 549 |
5 | 750 | 4,800 | 4,450 | 10,000 | 333 | 3,034 | 2,416 | 5,783 | 124 | 149 | 18 | 291 | 41 | 58 | 0 | 99 | 83 | 91 | 18 | 192 |
6 | 140 | 6,430 | 3,420 | 10,000 | 31 | 2,793 | 1485 | 4,309 | 6 | 118 | 16 | 140 | 1 | 28 | 4 | 33 | 5 | 90 | 12 | 107 |
7 | 480 | 4,760 | 4,760 | 10,000 | 48 | 493 | 615 | 1,156 | 22 | 165 | 42 | 229 | 0 | 17 | 1 | 18 | 22 | 148 | 41 | 211 |
Average | 753 | 4,541 | 4,705 | 10,000 | 153 | 1,788 | 1,600 | 3,541 | 178 | 247 | 64 | 489 | 18 | 37 | 6 | 61 | 159 | 210 | 58 | 427 |
C1* | 3,840 | 5,361 | 798 | 10,000 | 342 | 64 | 7 | 413 | ||||||||||||
C2† | 8,000 | 1,600 | 400 | 10,000 | 128 | 10 | 5 | 143 | ||||||||||||
C3‡ | 1,440 | 6,060 | 2,500 | 10,000 | 190 | 120 | 20 | 330 | ||||||||||||
Average | 4,427 | 4,340 | 1,233 | 10,000 | 220 | 65 | 11 | 295 |
Nine-week-old premature (28 weeks) infant, hematocrit level of 22%.
Ten-month-old infant with biliary atresia without hepatic failure, status post-Kasai procedure, anemia presumed secondary to documented gastrointestinal bleeding, hematocrit level of 27%.
Seven-week-old premature (30 weeks) infant, hematocrit level of 23%.