Induction of TNF-α Secretion After CD4XL With gp160 and Anti-gp160
| Treatment . | IL-2 Secretion . | TNF-α Secretion . |
|---|---|---|
| Medium | < | 65 ± 10 |
| Anti-CD3-GAM | 3,310 ± 210 | 2,548 ± 180 |
| gp160 | < | 600 ± 50 |
| gp160 + anti-gp160 | < | 811 ± 65 |
| Treatment . | IL-2 Secretion . | TNF-α Secretion . |
|---|---|---|
| Medium | < | 65 ± 10 |
| Anti-CD3-GAM | 3,310 ± 210 | 2,548 ± 180 |
| gp160 | < | 600 ± 50 |
| gp160 + anti-gp160 | < | 811 ± 65 |
CD4XL with gp160 and anti-gp160 was performed as described in Materials and Methods. Cell supernatants were harvested after 48 hours, and cytokine concentrations (in picograms per milliliter) were measured by ELISA using commercial kits according to manufacturer's instructions. < denotes undetectable levels.