In Vitro Effects of G-CSF on Proliferative Response of PBMNCs to Alloantigen Stimulation
| Experiment No. . | G-CSF (ng/mL) . | |||
|---|---|---|---|---|
| . | 0 . | 2 . | 10 . | 50 . |
| 1 | 3,841 (52) | 3,546 (920) | 4,967 (566) | 3,759 (632) |
| 2 | 7,368 (1,499) | 9,032 (2,915) | 9,913 (1,652) | 8,460 (400) |
| 3 | 4,861 (667) | 5,528 (570) | 5,603 (1,205) | 6,325 (1,036) |
| 4 | 6,785 (1,767) | 6,758 (1,010) | 7,168 (446) | 7,643 (1,399) |
| Experiment No. . | G-CSF (ng/mL) . | |||
|---|---|---|---|---|
| . | 0 . | 2 . | 10 . | 50 . |
| 1 | 3,841 (52) | 3,546 (920) | 4,967 (566) | 3,759 (632) |
| 2 | 7,368 (1,499) | 9,032 (2,915) | 9,913 (1,652) | 8,460 (400) |
| 3 | 4,861 (667) | 5,528 (570) | 5,603 (1,205) | 6,325 (1,036) |
| 4 | 6,785 (1,767) | 6,758 (1,010) | 7,168 (446) | 7,643 (1,399) |
Values are 3H-uptake (cpm). PBMNCs (1 × 105 cells) were cultured with the same number of irradiated allogeneic PBMNCs for 7 days in the absence or presence of various concentrations of G-CSF. Data are expressed as a mean (SD) of triplicates.