Number of CFU-MK, BFU-E, CFU-E, GM-CFC, CFU-mix, and CFU-GEMM Colonies Derived From BM Cells of Control Nontransgenic, HαIIb-tk, and MαIIb-tk Mice Treated With GCV
| . | CFU-MK . | BFU-E . | CFU-E . | GM-CFC . | CFU-mix . | CFU- . |
|---|---|---|---|---|---|---|
| . | . | . | . | . | . | GEMMK . |
| Control | 6 ± 2 | 10 ± 3 | 52 ± 12 | 62 ± 9 | 10 ± 3 | 7 ± 2 |
| HαIIb-tk | 1 ± 1 | 0 ± 1 | 46 ± 8 | 60 ± 6 | 0 ± 1 | 0 |
| MαIIb-tk | 1 ± 1 | 1 ± 1 | 37 ± 12 | 59 ± 4 | 0 ± 1 | 0 ± 1 |
| . | CFU-MK . | BFU-E . | CFU-E . | GM-CFC . | CFU-mix . | CFU- . |
|---|---|---|---|---|---|---|
| . | . | . | . | . | . | GEMMK . |
| Control | 6 ± 2 | 10 ± 3 | 52 ± 12 | 62 ± 9 | 10 ± 3 | 7 ± 2 |
| HαIIb-tk | 1 ± 1 | 0 ± 1 | 46 ± 8 | 60 ± 6 | 0 ± 1 | 0 |
| MαIIb-tk | 1 ± 1 | 1 ± 1 | 37 ± 12 | 59 ± 4 | 0 ± 1 | 0 ± 1 |
Marrow cells from control, HαIIb-tk (one copy of the transgene integrated) and MαIIb-tk (several copies integrated) mice treated with 0.1 mg of GCV/day/g body weight were obtained by femoral aspiration cultured as described in Materials and Methods. Each number (±SD) is the mean of three wells in three different experiments. CFU-mix colonies consisted of erythroid and megakaryocytic component, granulocytic, macrophagic, and erythroid component, or granulocytic macrophagic megakaryocytic without an erythroid component and multipotent CFU-GEMMK (granulocytic, erythrocytic macrophagic and megakaryocytic components).