GCV Sensitivity of tk Nonexpressing Cells in the Presence of tk Expressing Cells
| . | MK . | GM . | BFU-E . | ||||||
|---|---|---|---|---|---|---|---|---|---|
| . | . | . | . | . | . | . | . | . | . |
| GCV | − | + | ± | − | + | ± | − | + | ± |
| 0 | 19 ± 2 | 10 ± 1 | 16 ± 2 | 36 ± 3 | 32 ± 4 | 28 ± 4 | 11 ± 2 | 15 ± 2 | 13 ± 2 |
| 5 μmol/L | 17 ± 2 | 0 | 8 ± 1 | 26 ± 1 | 0 | 25 ± 3 | 10 ± 1 | 0 | 6 ± 1 |
| . | MK . | GM . | BFU-E . | ||||||
|---|---|---|---|---|---|---|---|---|---|
| . | . | . | . | . | . | . | . | . | . |
| GCV | − | + | ± | − | + | ± | − | + | ± |
| 0 | 19 ± 2 | 10 ± 1 | 16 ± 2 | 36 ± 3 | 32 ± 4 | 28 ± 4 | 11 ± 2 | 15 ± 2 | 13 ± 2 |
| 5 μmol/L | 17 ± 2 | 0 | 8 ± 1 | 26 ± 1 | 0 | 25 ± 3 | 10 ± 1 | 0 | 6 ± 1 |
BM cells (1 × 105) of transgenic (+) mice or nontransgenic littermates (−) were plated in the presence of 5 μmol/L GCV or in its absence and cultured for 6 days. For the mixed-cultures (±), 75,000 cells/mL of nontransgenic BM cells were cultured in the same well as 75,000 cells/mL of transgenic cells.