Transactivation of the CD20 Gene Promoter
| Transcription Factor(s) . | Induction Wild Type* . | Induction of Mutated Construct† . |
|---|---|---|
| PU.1 | 1 | 1 |
| Pip | 1 | 1 |
| PU.1 + Pip | 2.3 | 1 |
| Oct-2 | 2.2 | NT |
| TFE3 | 2 | 1 |
| USF | 6.5 | 1.6 |
| PU.1 + Pip + Oct-2 | 4.4 | NT |
| PU.1 + Pip + TFE3 | 4 | NT |
| PU.1 + Pip + USF | 6.5 | NT |
| USF + Oct-2 | 9.3 | NT |
| Transcription Factor(s) . | Induction Wild Type* . | Induction of Mutated Construct† . |
|---|---|---|
| PU.1 | 1 | 1 |
| Pip | 1 | 1 |
| PU.1 + Pip | 2.3 | 1 |
| Oct-2 | 2.2 | NT |
| TFE3 | 2 | 1 |
| USF | 6.5 | 1.6 |
| PU.1 + Pip + Oct-2 | 4.4 | NT |
| PU.1 + Pip + TFE3 | 4 | NT |
| PU.1 + Pip + USF | 6.5 | NT |
| USF + Oct-2 | 9.3 | NT |
A total of 6 μg of construct pGL3-CD20-425 were transiently cotransfected with 2.5 μg of each indicated expression vector into NIH-3T3 fibroblasts. The total amount of transfected DNA was kept constant with empty expression vector pC-CMV and a β-Gal control plasmid was cotransfected to normalize for transfection efficiency. Cells were harvested and luciferase assay performed after 16 hours.
The Luciferase Activity of the reporter construct alone has been set at 1.0. Induction refers to fold increase of luciferase activity observed with cotransfection. Results reported are an average of 3 to 5 independent transfections.
For control experiments a promoter-reporter construct with a mutation of the PU.1/Pip or μE3 binding site was used.