Marrow Analysis in Leukemic Bone Biopsies Engrafted in SCID Mice for Two Different Periods
| . | Human BM Biopsy . | |||
|---|---|---|---|---|
| . | R43 . | R44 . | R48 . | R49 . |
| First graft harvest | ||||
| Engraftment period | 6 wk | 5 wk | 6 wk | 6 wk |
| Cytology | 98% CD19+ lymphoblasts | 88% CD19+ lymphoblasts | 27% CD19+ lymphoblasts | 92% CD19+ lymphoblasts |
| Histology | Diffuse blastic infiltration; myelofibrosis; areas of necrosis | Diffuse blastic infiltration; myelofibrosis; areas of necrosis | Myelofibrosis; small foci of blastic infiltration | Diffuse blastic infiltration; myelofibrosis |
| Second graft harvest | ||||
| Engraftment period | 12 wk | 12 wk | 9 wk | 9 wk |
| Cytology | 85% CD19+ lymphoblasts | 90% CD19+ lymphoblasts | Rare blasts | 52% CD19+ lymphoblasts |
| Histology | Diffuse blastic infiltration; myelofibrosis | Diffuse blastic infiltration; myelofibrosis | Small foci of blastic infiltration; myelofibrosis | Blast dissemination in surrounding murine tissues (muscle, nerve); diffuse blastic infiltration; myelofibrosis |
| . | Human BM Biopsy . | |||
|---|---|---|---|---|
| . | R43 . | R44 . | R48 . | R49 . |
| First graft harvest | ||||
| Engraftment period | 6 wk | 5 wk | 6 wk | 6 wk |
| Cytology | 98% CD19+ lymphoblasts | 88% CD19+ lymphoblasts | 27% CD19+ lymphoblasts | 92% CD19+ lymphoblasts |
| Histology | Diffuse blastic infiltration; myelofibrosis; areas of necrosis | Diffuse blastic infiltration; myelofibrosis; areas of necrosis | Myelofibrosis; small foci of blastic infiltration | Diffuse blastic infiltration; myelofibrosis |
| Second graft harvest | ||||
| Engraftment period | 12 wk | 12 wk | 9 wk | 9 wk |
| Cytology | 85% CD19+ lymphoblasts | 90% CD19+ lymphoblasts | Rare blasts | 52% CD19+ lymphoblasts |
| Histology | Diffuse blastic infiltration; myelofibrosis | Diffuse blastic infiltration; myelofibrosis | Small foci of blastic infiltration; myelofibrosis | Blast dissemination in surrounding murine tissues (muscle, nerve); diffuse blastic infiltration; myelofibrosis |