SCL Expression During Erythroid Differentiation in Normal Donors and DBA
| SCF . | Quantity (OD × mm) . | |||||
|---|---|---|---|---|---|---|
| . | Day 10 . | Day 14 . | n . | . | ||
| . | − . | + . | − . | + . | . | . |
| Normal donors | 0.084 ± 0.013 | 0.137 ± 0.021* | 0.055 ± 0.012 | 0.055 ± 0.007 | 6 | |
| DBA | 0.080 ± 0.012 | 0.122 ± 0.019* | 0.044 ± 0.010 | 0.058 ± 0.008 | 6 | |
| SCF . | Quantity (OD × mm) . | |||||
|---|---|---|---|---|---|---|
| . | Day 10 . | Day 14 . | n . | . | ||
| . | − . | + . | − . | + . | . | . |
| Normal donors | 0.084 ± 0.013 | 0.137 ± 0.021* | 0.055 ± 0.012 | 0.055 ± 0.007 | 6 | |
| DBA | 0.080 ± 0.012 | 0.122 ± 0.019* | 0.044 ± 0.010 | 0.058 ± 0.008 | 6 | |
PB BFU-E from normal donors or patients with DBA were cultured with or without 100 ng/mL human SCF and colonies removed from culture on day 10 and 14. The cell lysate from 1 × 105 BFU-E derived cells was loaded onto each lane of a 10% polyacrylamide gel and immunoblotting performed with anti-SCL antibody. The mean ± SEM density of bands at 45 kD and 47 kD quantitated with a Molecular Dynamics Densitometer and Quantity One Software (Huntington, NY) is shown here. n = number of individuals studied.
A significant increase in SCL expression was observed in response to stem cell factor stimulation at day 10 (P < .05).