Exocytosis of Granule Constituents in Response to Stimulation of Neutrophils
| . | Gelatinase (%) . | hCAP-18 (%) . | Lactoferrin (%) . | Myeloperoxidase (%) . |
|---|---|---|---|---|
| No addition | 4.7 | 2.3 | 2.5 | 1.5 |
| fMLP, 10−8 mol/L | 20.2 | 2.9 | 3.0 | 1.5 |
| Ionomycin, 1 μmol/L | 89.5 | 58.8 | 53.6 | 21.6 |
| STZ, 1 mg/mL | 22.9 | 7.6 | 8.8 | 3.6 |
| . | Gelatinase (%) . | hCAP-18 (%) . | Lactoferrin (%) . | Myeloperoxidase (%) . |
|---|---|---|---|---|
| No addition | 4.7 | 2.3 | 2.5 | 1.5 |
| fMLP, 10−8 mol/L | 20.2 | 2.9 | 3.0 | 1.5 |
| Ionomycin, 1 μmol/L | 89.5 | 58.8 | 53.6 | 21.6 |
| STZ, 1 mg/mL | 22.9 | 7.6 | 8.8 | 3.6 |
Exocytosis of granule constituents in response to stimulation of neutrophils. Isolated cells were incubated with or without stimulus. The amounts of granule proteins exocytosed into medium and amounts retained in cells was determined. Exocytosis is expressed as % of total amount present in cells and medium. Results are given as mean of four independent experiments except for Ionomycin stimulation where only 3 experiments were performed.