Table 2. Colocalization of hCAP-18 and... | American Society of Hematology

Table 2.

Colocalization of hCAP-18 and Lactoferrin in Granules of Human Neutrophils Examined by Double Labeling Immuno-Electron Microscopy

hCAP-18 10 nm Gold Lactoferrin 15 nm Gold	h-CAP-18 Single Label	Lactoferrin Single Label	h-CAP-18 and Lactoferrin Double Label

% of granules counted	11.6	40.8	47.5
% of gold particles counted	6	21	73
h-CAP-18 15 nm gold
Lactoferrin 10 nm gold
% of granules counted	10.9	38.4	50.7
% of gold particles counted	3	34	63

hCAP-18 10 nm Gold Lactoferrin 15 nm Gold	h-CAP-18 Single Label	Lactoferrin Single Label	h-CAP-18 and Lactoferrin Double Label

% of granules counted	11.6	40.8	47.5
% of gold particles counted	6	21	73
h-CAP-18 15 nm gold
Lactoferrin 10 nm gold
% of granules counted	10.9	38.4	50.7
% of gold particles counted	3	34	63

Colocalization of hCAP-18 and lactoferrin in granules of human neutrophils examined by double labeling immuno-electron microscopy. Twenty-five micrographs of neutrophils double labeled with biotinylated hCAP-18 antibody, streptavidin gold 10 nm, followed by lactoferrin antibody and goat antirabbit gold 15 nm were examined along with 25 micrographs of neutrophils double labeled with hCAP-18 antibody, goat antirabbit gold 15 nm, followed by biotinylated lactoferrin antibody, streptavidin gold 10 nm. Only labeled granules were counted.

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