MRD as Assessed by Detection of Cells Bearing a BCL2 and/or a IgH Gene Rearrangement on BM (patients 11 to 32) and PBPC (patients 34 to 48) Before (buffy coat) and After CD34 Selection (selected)
| Patient No. . | Histology . | BM Involvement at Diagnosis . | MRD . | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| . | . | . | BCL2/JH . | IgH . | Phenotype . | . | |||||||||
| . | . | . | BC . | Selected . | BC . | Selected . | BC . | Selected . | . | . | . | . | |||
| . | . | . | . | . | . | . | CD34 . | CD19 . | CD34 . | CD19 . | CD34+/19+ . | . | . | . | . |
| . | . | . | . | . | . | . | (%) . | (%) . | (%) . | (%) . | (%) . | . | . | . | . |
| 11 | F | + | + | − | − | − | 1.8 | 1.5 | 40 | 41.2 | 14.0 | ||||
| 14 | F | + | + | + | − | − | 2.2 | 2.0 | 81 | 3.0 | 2.2 | ||||
| 15 | F | + | + | − | − | − | 0.7 | 10.0 | 32 | 7.4 | 7.0 | ||||
| 19 | F | + | + | − | − | − | 0.5 | 0.4 | 28 | 6.1 | 5.4 | ||||
| 20 | F | + | + | − | − | − | 0.7 | 1.0 | 34 | 3.0 | 2.9 | ||||
| 22 | F | + | + | − | − | − | 0.8 | 0.8 | 68 | 1.0 | ND | ||||
| 23 | DIFF | − | + | − | − | − | 0.9 | 2.3 | 59 | 23.5 | 21.1 | ||||
| 24 | F | + | + | − | − | − | 0.5 | 1.7 | 40 | 9.0 | 9.0 | ||||
| 25 | F | + | − | − | + | + | 1.4 | 4.2 | 68 | 17.9 | 15.0 | ||||
| 28 | F | − | + | + | − | − | 1.1 | ND | 51 | 48.3 | 43 | ||||
| 32 | F | + | + | + | − | − | 0.6 | 0.3 | 69 | ND | ND | ||||
| 34 | F | + | − | − | + | − | 0.8 | 6.0 | 16 | 14.0 | 11.7 | ||||
| 35 | HG | + | − | − | + | + | 2.0 | 0.9 | 81 | ND | ND | ||||
| 36 | F | + | − | − | + | − | 0.6 | 0.6 | 55 | 43.0 | 11.0 | ||||
| 37 | HG | − | − | − | + | + | 2.2 | 0.3 | 76 | 0.4 | 0.3 | ||||
| 38 | F | + | − | − | + | + | 0.4 | 1.9 | 54 | 2.0 | 1.6 | ||||
| 39 | F | + | − | − | + | + | 1.3 | 4.0 | 58 | 51.0 | 50.0 | ||||
| 40 | HG | − | − | − | + | + | 1.2 | ND | 78 | ND | 1.5 | ||||
| 41 | F | + | − | − | + | − | 1.1 | 0.9 | 85 | 1.0 | 0.6 | ||||
| 42 | F | + | − | − | + | + | 0.8 | 12.5 | 69 | 4.0 | 1.0 | ||||
| 43 | F | + | − | − | + | + | 1.1 | 0.8 | 72 | 1.4 | 1.1 | ||||
| 44 | F | + | − | − | + | + | 1.0 | 2.0 | 91 | 2.0 | 2.0 | ||||
| 45 | F | + | + | + | − | − | 1.4 | ND | 17 | 14.0 | ND | ||||
| 46 | HG | − | − | − | + | + | 0.8 | 1.3 | 73 | 2.0 | 2.0 | ||||
| 47 | F | + | + | + | + | + | 0.7 | 1.1 | 99 | 0.4 | 0.3 | ||||
| 48 | F | + | − | − | + | + | 0.3 | 8.0 | 22 | 19.0 | 15.5 | ||||
| Patient No. . | Histology . | BM Involvement at Diagnosis . | MRD . | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| . | . | . | BCL2/JH . | IgH . | Phenotype . | . | |||||||||
| . | . | . | BC . | Selected . | BC . | Selected . | BC . | Selected . | . | . | . | . | |||
| . | . | . | . | . | . | . | CD34 . | CD19 . | CD34 . | CD19 . | CD34+/19+ . | . | . | . | . |
| . | . | . | . | . | . | . | (%) . | (%) . | (%) . | (%) . | (%) . | . | . | . | . |
| 11 | F | + | + | − | − | − | 1.8 | 1.5 | 40 | 41.2 | 14.0 | ||||
| 14 | F | + | + | + | − | − | 2.2 | 2.0 | 81 | 3.0 | 2.2 | ||||
| 15 | F | + | + | − | − | − | 0.7 | 10.0 | 32 | 7.4 | 7.0 | ||||
| 19 | F | + | + | − | − | − | 0.5 | 0.4 | 28 | 6.1 | 5.4 | ||||
| 20 | F | + | + | − | − | − | 0.7 | 1.0 | 34 | 3.0 | 2.9 | ||||
| 22 | F | + | + | − | − | − | 0.8 | 0.8 | 68 | 1.0 | ND | ||||
| 23 | DIFF | − | + | − | − | − | 0.9 | 2.3 | 59 | 23.5 | 21.1 | ||||
| 24 | F | + | + | − | − | − | 0.5 | 1.7 | 40 | 9.0 | 9.0 | ||||
| 25 | F | + | − | − | + | + | 1.4 | 4.2 | 68 | 17.9 | 15.0 | ||||
| 28 | F | − | + | + | − | − | 1.1 | ND | 51 | 48.3 | 43 | ||||
| 32 | F | + | + | + | − | − | 0.6 | 0.3 | 69 | ND | ND | ||||
| 34 | F | + | − | − | + | − | 0.8 | 6.0 | 16 | 14.0 | 11.7 | ||||
| 35 | HG | + | − | − | + | + | 2.0 | 0.9 | 81 | ND | ND | ||||
| 36 | F | + | − | − | + | − | 0.6 | 0.6 | 55 | 43.0 | 11.0 | ||||
| 37 | HG | − | − | − | + | + | 2.2 | 0.3 | 76 | 0.4 | 0.3 | ||||
| 38 | F | + | − | − | + | + | 0.4 | 1.9 | 54 | 2.0 | 1.6 | ||||
| 39 | F | + | − | − | + | + | 1.3 | 4.0 | 58 | 51.0 | 50.0 | ||||
| 40 | HG | − | − | − | + | + | 1.2 | ND | 78 | ND | 1.5 | ||||
| 41 | F | + | − | − | + | − | 1.1 | 0.9 | 85 | 1.0 | 0.6 | ||||
| 42 | F | + | − | − | + | + | 0.8 | 12.5 | 69 | 4.0 | 1.0 | ||||
| 43 | F | + | − | − | + | + | 1.1 | 0.8 | 72 | 1.4 | 1.1 | ||||
| 44 | F | + | − | − | + | + | 1.0 | 2.0 | 91 | 2.0 | 2.0 | ||||
| 45 | F | + | + | + | − | − | 1.4 | ND | 17 | 14.0 | ND | ||||
| 46 | HG | − | − | − | + | + | 0.8 | 1.3 | 73 | 2.0 | 2.0 | ||||
| 47 | F | + | + | + | + | + | 0.7 | 1.1 | 99 | 0.4 | 0.3 | ||||
| 48 | F | + | − | − | + | + | 0.3 | 8.0 | 22 | 19.0 | 15.5 | ||||
Abbreviations: MRD, minimal residual disease; F, follicular; HG, high grade; DIFF, diffuse; ND, not done.