Cellular Composition of Stem Cell Transplants
| Strain . | Source . | n . | Leukocytes (/nL) . | Neutrophils (%) . | MNC (%) . | T Cells CD3+ (%) . | NK Cells 5E6+ (%) . | B Cells CD19+ (%) . | Progenitor Cells CFU-GM . |
|---|---|---|---|---|---|---|---|---|---|
| . | . | . | . | . | . | . | . | . | (/104 cells) . |
| Balb/c | PB | 4 | 4.9 (3.4-8.2) | 10 (10-50) | 90 | 21 (11-32) | 7 (4-11) | ND | 0.13 (0.06-0.2) |
| PBPC | 6 | 59.6 (42.6-73.3) | 50 (20-80) | 50 | 32 (15-35) | 4 (2-5) | 11 (4-13) | 0.53 (0.2-0.87) | |
| DBA/2 | PB | 6 | 9.9 (9.4-10.8) | 50 (10-60) | 50 | 20 (12-27) | 11 (8-13) | ND | 0.80 (0.67-3.0) |
| PBPC | 6 | 72.9 (51.8-85.8) | 40 (15-60) | 60 | 62 (50-71) | 5 (2-7) | 20 (11-24) | 1.13 (0.07-3.6) | |
| BM | 6 | ND | ND | ND | 14 (6-30) | 4 (3-4) | ND | 4.83 (1.4-11.47) |
| Strain . | Source . | n . | Leukocytes (/nL) . | Neutrophils (%) . | MNC (%) . | T Cells CD3+ (%) . | NK Cells 5E6+ (%) . | B Cells CD19+ (%) . | Progenitor Cells CFU-GM . |
|---|---|---|---|---|---|---|---|---|---|
| . | . | . | . | . | . | . | . | . | (/104 cells) . |
| Balb/c | PB | 4 | 4.9 (3.4-8.2) | 10 (10-50) | 90 | 21 (11-32) | 7 (4-11) | ND | 0.13 (0.06-0.2) |
| PBPC | 6 | 59.6 (42.6-73.3) | 50 (20-80) | 50 | 32 (15-35) | 4 (2-5) | 11 (4-13) | 0.53 (0.2-0.87) | |
| DBA/2 | PB | 6 | 9.9 (9.4-10.8) | 50 (10-60) | 50 | 20 (12-27) | 11 (8-13) | ND | 0.80 (0.67-3.0) |
| PBPC | 6 | 72.9 (51.8-85.8) | 40 (15-60) | 60 | 62 (50-71) | 5 (2-7) | 20 (11-24) | 1.13 (0.07-3.6) | |
| BM | 6 | ND | ND | ND | 14 (6-30) | 4 (3-4) | ND | 4.83 (1.4-11.47) |
Characterization of stem cell transplants as administered to leukemia-bearing Balb/c recipients. Medians and ranges are given for data obtained from the pooled grafts derived from 1 or 2 donors. The flow cytometry analysis was performed in parallel in 6 of 10 single transplantation experiments. The percentage of lymphocytes is given as the percentage of nucleated cells. Nucleated cells were also the basis for the calculation of graft size in all experiments. The MoAb 5E6 labels a subpopulation of NK cells. CFU-GM were counted on day +12 after seeding.
Abbreviations: MNC, mononuclear cells; ND, not determined.