Tissue Distribution of Neutrophils and Hematopoietic Progenitors at Baseline or Following Cyclophosphamide or flt-3 Ligand Treatment
| Tissue . | G-CSFR Genotype . | Baseline . | Cyclophosphamide . | Flt-3 Ligand . | |||
|---|---|---|---|---|---|---|---|
| . | . | PMN (×10−6) . | CFU-C . | PMN (×10E-6) . | CFU-C . | PMN (×10−6) . | CFU-C . |
| Blood (per mL) | (+/+) | 0.79 ± 0.10 | 131 ± 28 | 6.47 ± 1.24 | 8,100 ± 2,730 | 7.12 ± 2.21 | 38,000 ± 6,100 |
| (−/−) | 0.12 ± 0.01* | 178 ± 24 | 0.18 ± 0.04* | 67 ± 28‡ | 1.72 ± 0.32 | 21,000 ± 4,200 | |
| BM (per femur) | (+/+) | 5.09 ± 0.58 | 96,262 ± 3,989 | 18.68 ± 1.94 | 132,525 ± 24,350 | 8.23 ± 0.48 | 254,338 ± 49,058 |
| (−/−) | 2.33 ± 0.44† | 79,525 ± 8,569 | 5.46 ± 1.12† | 222,502 ± 27,983ρ | 3.72 ± 2.45 | 127,188 ± 9,643 | |
| Spleen (total) | (+/+) | ND | 22,240 ± 3,705 | ND | 208,592 ± 32,000 | ND | 945,000 ± 81,624 |
| (−/−) | ND | 22,124 ± 5,587 | ND | 11,029 ± 2,846* | ND | 474,525 ± 90,302ρ | |
| Total (per mouse) | (+/+) | ND | 1,626,638 ± 67,975 | ND | 2,431,916 ± 400,241 | ND | 4,912,715 ± 772,881 |
| (−/−) | ND | 1,348,890 ± 146,111 | ND | 3,719,515 ± 467,286 | ND | 2,559,328 ± 216,829ρ | |
| Tissue . | G-CSFR Genotype . | Baseline . | Cyclophosphamide . | Flt-3 Ligand . | |||
|---|---|---|---|---|---|---|---|
| . | . | PMN (×10−6) . | CFU-C . | PMN (×10E-6) . | CFU-C . | PMN (×10−6) . | CFU-C . |
| Blood (per mL) | (+/+) | 0.79 ± 0.10 | 131 ± 28 | 6.47 ± 1.24 | 8,100 ± 2,730 | 7.12 ± 2.21 | 38,000 ± 6,100 |
| (−/−) | 0.12 ± 0.01* | 178 ± 24 | 0.18 ± 0.04* | 67 ± 28‡ | 1.72 ± 0.32 | 21,000 ± 4,200 | |
| BM (per femur) | (+/+) | 5.09 ± 0.58 | 96,262 ± 3,989 | 18.68 ± 1.94 | 132,525 ± 24,350 | 8.23 ± 0.48 | 254,338 ± 49,058 |
| (−/−) | 2.33 ± 0.44† | 79,525 ± 8,569 | 5.46 ± 1.12† | 222,502 ± 27,983ρ | 3.72 ± 2.45 | 127,188 ± 9,643 | |
| Spleen (total) | (+/+) | ND | 22,240 ± 3,705 | ND | 208,592 ± 32,000 | ND | 945,000 ± 81,624 |
| (−/−) | ND | 22,124 ± 5,587 | ND | 11,029 ± 2,846* | ND | 474,525 ± 90,302ρ | |
| Total (per mouse) | (+/+) | ND | 1,626,638 ± 67,975 | ND | 2,431,916 ± 400,241 | ND | 4,912,715 ± 772,881 |
| (−/−) | ND | 1,348,890 ± 146,111 | ND | 3,719,515 ± 467,286 | ND | 2,559,328 ± 216,829ρ | |
Band, ring, and polymorphonuclear neutrophils were scored as mature neutrophils (PMN). Total body CFU-C were calculated by assuming a blood volume of 1.8 mL and a whole femur equivalent to 6% of the total BM. Analyses were performed at baseline, 8 days after a single intraperitoneal injection of cyclophosphamide, or after 10 days of flt-3 ligand administration. Five to 6 age- and sex-matched mice were analyzed for each data entry. Data represent the mean ± SEM.
Abbreviation: ND, not done.
Statistical comparison to wild type: * P < .001; † P < .01; ‡ P < .02; ρ P < .05.