Production of MCP-1 by Human Monocytes Upon Stimulation by IL-15
| Treatment* . | MCP-1 (ng/mL) . | |
|---|---|---|
| . | Mean (SD) . | |
| . | 6 h . | 24 h . |
| Medium | 0.16 (0.1) | <0.014 (0.01) |
| IL-15 (10 ng/mL) | 0.44 (0.2) | 0.05 (0.09) |
| (100 ng/mL) | 0.78† (0.16) | 0.14 (0.12) |
| (1,000 ng/mL) | 1.85† (0.31) | 1.03† (0.2) |
| Treatment* . | MCP-1 (ng/mL) . | |
|---|---|---|
| . | Mean (SD) . | |
| . | 6 h . | 24 h . |
| Medium | 0.16 (0.1) | <0.014 (0.01) |
| IL-15 (10 ng/mL) | 0.44 (0.2) | 0.05 (0.09) |
| (100 ng/mL) | 0.78† (0.16) | 0.14 (0.12) |
| (1,000 ng/mL) | 1.85† (0.31) | 1.03† (0.2) |
Supernatants derived from monocytes cultured with increasing concentrations of IL-15 (from 10 to 1,000 ng/mL), or medium alone for 6 or 24 hours were analyzed in duplicate for their concentration of MCP-1 by ELISA. Results shown are representative of a single experiment out of three performed.
Significant production in comparison to medium (P < .05).