Table 1. Fractions of Ca 2 -Responsive... | American Society of Hematology

Table 1.

Fractions of Ca²⁺-Responsive and Annexin V-Binding Platelets After Adhesion to Fibrinogen or Collagen Matrices

			Increase in	Binding of
			[Ca²⁺]_i	Annexin V
Coverslip	Thrombin	No. of

Coating	(nmol/L)	Analyzed Cells	(% of analyzed cells)

Fibrinogen	0	310	11.9	1.6
	2	290	87.6	2.8
Collagen	0	410	56.8	57.8
	2	120	75.8	80.0
None^*	0	125	6.9	2.6

			Increase in	Binding of
			[Ca²⁺]_i	Annexin V
Coverslip	Thrombin	No. of

Coating	(nmol/L)	Analyzed Cells	(% of analyzed cells)

Fibrinogen	0	310	11.9	1.6
	2	290	87.6	2.8
Collagen	0	410	56.8	57.8
	2	120	75.8	80.0
None^*	0	125	6.9	2.6

Fura-2–loaded platelets were allowed to adhere to fibrinogen-or collagen-coated surfaces in the presence of 2 mmol/L CaCl₂ and FITC-labeled annexin V during 20 minutes. Thrombin was added after 5 minutes of incubation where indicated. Images of fura-2 fluorescence ratio and FITC-fluorescence intensity were then recorded from the same microscopic field, and percentages of platelets with elevated [Ca²⁺]_i were determined as well as the percentages of the FITC-positive cells. Cumulated data are given from 3 to 4 independent experiments.

Coverslips were prewashed with HEPES buffer pH 7.4 containing 0.1% (wt/vol) bovine serum albumin.

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