Radioactivity of rTFPI in Serum and Fibrin Clot From Normal, AT- and Plasminogen-Depleted, or Factor XIII–Deficient Plasma
| Plasma . | Radioactivity (% of total count) . | |||||
|---|---|---|---|---|---|---|
| . | After 15 min . | After 20 h . | . | . | ||
| . | Serum . | Clot . | Serum . | Clot . | . | . |
| rTFPI | ||||||
| Normal (n = 9) | 33.9 ± 1.1 | 63.5 ± 1.7 | 51.2 ± 1.4 | 48.0 ± 1.4 | ||
| AT-depleted (n = 6) | 37.7 ± 1.6 | 60.3 ± 1.5 | 64.8 ± 2.2 | 33.5 ± 2.8 | ||
| Plasminogen-depleted (n = 6) | 36.6 ± 0.5 | 61.1 ± 0.9 | 53.9 ± 0.9 | 44.2 ± 1.0 | ||
| AT- and plasminogen-depleted (n = 6) | 39.3 ± 0.8 | 59.5 ± 0.9 | 66.2 ± 1.4 | 33.1 ± 1.5 | ||
| Factor XIII–deficient (n = 6) | 36.3 ± 1.2 | 60.3 ± 0.3 | ||||
| rTFPI-C | ||||||
| Normal (n = 7) | 78.3 ± 2.8 | 20.6 ± 2.9 | ||||
| Plasma . | Radioactivity (% of total count) . | |||||
|---|---|---|---|---|---|---|
| . | After 15 min . | After 20 h . | . | . | ||
| . | Serum . | Clot . | Serum . | Clot . | . | . |
| rTFPI | ||||||
| Normal (n = 9) | 33.9 ± 1.1 | 63.5 ± 1.7 | 51.2 ± 1.4 | 48.0 ± 1.4 | ||
| AT-depleted (n = 6) | 37.7 ± 1.6 | 60.3 ± 1.5 | 64.8 ± 2.2 | 33.5 ± 2.8 | ||
| Plasminogen-depleted (n = 6) | 36.6 ± 0.5 | 61.1 ± 0.9 | 53.9 ± 0.9 | 44.2 ± 1.0 | ||
| AT- and plasminogen-depleted (n = 6) | 39.3 ± 0.8 | 59.5 ± 0.9 | 66.2 ± 1.4 | 33.1 ± 1.5 | ||
| Factor XIII–deficient (n = 6) | 36.3 ± 1.2 | 60.3 ± 0.3 | ||||
| rTFPI-C | ||||||
| Normal (n = 7) | 78.3 ± 2.8 | 20.6 ± 2.9 | ||||
Radiolabeled rTFPI was mixed with plasma, and coagulation was induced by addition of TF and calcium chloride. In the case of normal plasma, both rTFPI and rTFPI-C were used. After 15 minutes or 20 hours, the serum and fibrin clot were separated. The fibrin clot was washed with TBS. Data represent the mean ± SD.