Effect of Adenovirus-Mediated Gene Transfer on Incidence and Severity of Liver Metastases in Athymic Nude Mice Bearing Intraocular Melanomas
| Tumor . | Treatment . | Liver Metastases . | |||||||
|---|---|---|---|---|---|---|---|---|---|
| . | . | Incidence . | P . | Severity of Metastatic Foci . | P . | . | . | ||
| . | . | . | . | 1+ . | 2+ . | 3+ . | . | . | . |
| OCM-1 | None | 14/24 (58%) | — | 1 | 5 | 8 | — | ||
| OCM-1 | AdCMV-PAI-1 (1× IC)* | 6/19 (31%) | >.05† | 2 | 2 | 2 | .06‡ | ||
| OCM-1 | None | 14/19 (74%) | — | 3 | 5 | 6 | — | ||
| OCM-1 | AdCMV-βGal (2× IC) | 14/20 (70%) | >.05 | 2 | 4 | 8 | >.05 | ||
| OCM-1 | AdCMV-PAI-1 (2× IC) | 6/20 (30%) | .01 | 2 | 3 | 1 | .005 | ||
| OCM-1 | None | 7/10 (70%) | — | 2 | 1 | 4 | — | ||
| OCM-1 | AdCMV-βGal (2× IV) | 7/10 (70%) | >.05 | 2 | 2 | 3 | >.05 | ||
| OCM-1 | AdCMV-PAI-1 (2× IV) | 5/10 (50%) | >.05 | 2 | 1 | 2 | >.05 | ||
| OCM-1 | None | 13/20 (65%) | — | 3 | 2 | 8 | — | ||
| OCM-1 | AdCMV-βGal (3× IV) | 14/20 (70%) | >.05 | 2 | 6 | 6 | >.05 | ||
| OCM-1 | AdCMV-PAI-1 (3× IV) | 5/20 (25%) | .02 | 3 | 1 | 1 | .003 | ||
| 99E1 | None | 6/10 (60%) | — | 3 | 3 | 0 | — | ||
| 99E1 | AdCMV-PAI-1 (1× IC) | 3/10 (30%) | >.05 | 2 | 1 | 0 | >.05 | ||
| 99E1 | None | 14/19 (74%) | — | 3 | 2 | 9 | — | ||
| 99E1 | AdCMV-βGAL | 13/20 (65%) | >.05 | 1 | 3 | 9 | >.05 | ||
| 99E1 | AdCMV-PAI-1 (2× IC) | 6/20 (30%) | .01 | 4 | 0 | 2 | .002 | ||
| Tumor . | Treatment . | Liver Metastases . | |||||||
|---|---|---|---|---|---|---|---|---|---|
| . | . | Incidence . | P . | Severity of Metastatic Foci . | P . | . | . | ||
| . | . | . | . | 1+ . | 2+ . | 3+ . | . | . | . |
| OCM-1 | None | 14/24 (58%) | — | 1 | 5 | 8 | — | ||
| OCM-1 | AdCMV-PAI-1 (1× IC)* | 6/19 (31%) | >.05† | 2 | 2 | 2 | .06‡ | ||
| OCM-1 | None | 14/19 (74%) | — | 3 | 5 | 6 | — | ||
| OCM-1 | AdCMV-βGal (2× IC) | 14/20 (70%) | >.05 | 2 | 4 | 8 | >.05 | ||
| OCM-1 | AdCMV-PAI-1 (2× IC) | 6/20 (30%) | .01 | 2 | 3 | 1 | .005 | ||
| OCM-1 | None | 7/10 (70%) | — | 2 | 1 | 4 | — | ||
| OCM-1 | AdCMV-βGal (2× IV) | 7/10 (70%) | >.05 | 2 | 2 | 3 | >.05 | ||
| OCM-1 | AdCMV-PAI-1 (2× IV) | 5/10 (50%) | >.05 | 2 | 1 | 2 | >.05 | ||
| OCM-1 | None | 13/20 (65%) | — | 3 | 2 | 8 | — | ||
| OCM-1 | AdCMV-βGal (3× IV) | 14/20 (70%) | >.05 | 2 | 6 | 6 | >.05 | ||
| OCM-1 | AdCMV-PAI-1 (3× IV) | 5/20 (25%) | .02 | 3 | 1 | 1 | .003 | ||
| 99E1 | None | 6/10 (60%) | — | 3 | 3 | 0 | — | ||
| 99E1 | AdCMV-PAI-1 (1× IC) | 3/10 (30%) | >.05 | 2 | 1 | 0 | >.05 | ||
| 99E1 | None | 14/19 (74%) | — | 3 | 2 | 9 | — | ||
| 99E1 | AdCMV-βGAL | 13/20 (65%) | >.05 | 1 | 3 | 9 | >.05 | ||
| 99E1 | AdCMV-PAI-1 (2× IC) | 6/20 (30%) | .01 | 4 | 0 | 2 | .002 | ||
AdCMV-PAI-1 or AdCMV-βGal were inoculated IC either on day 7 or days 7 and 9. IV inocula containing either AdCMV-βGal or AdCMV-PAI-1 were administered on days 21 and 28 or on days 21, 28, and 35. Mice were euthanized on day 49 and liver metastases were evaluated histopathologically by three independent observers.
Determined by chi square analysis
Determined by Cochran-Mantel-Haenszel test