PCR Primers for Amplification of Exons in the HRG Gene
| Primer . | Region . | Direction . | Sequence . | Length of Product (bp) . |
|---|---|---|---|---|
| Pair 1 | Exon 1 | Upstream | 5′-CGGAATTCTCTGCAGTGGCAGATC-3′ | 256 |
| Downstream | 5′-CGGGATCCTCTGCCATTGGCAATTCC-3′ | |||
| Pair 2 | Exon 2 | Upstream | 5′-CGGAATTCACATGTTGCTTTTGGC-3′ | 184 |
| Downstream | 5′-CGGGATCCAGAGTGAAGGTGCCCTC-3′ | |||
| Pair 3 | Exon 3 | Upstream | 5′-CGGAATTCCTCAAGAGCCTCTTTC-3′ | 149 |
| Downstream | 5′-CGGGATCCAATTTAACAGATTACC-3′ | |||
| Pair 4 | Exon 4 | Upstream | 5′-CGGAATTCACTGCTATCTTCTTTC-3′ | 224 |
| Downstream | 5′-CGGGATCCGAACCTTAGTGGAGAC-3′ | |||
| Pair 5 | Exon 5 | Upstream | 5′-CGGAATTCTTGAAACTATTTGATCCC-3′ | 155 |
| Downstream | 5′-CGGGATCCAACGATCACAGACATTTC-3′ | |||
| Pair 6 | Exon 6 | Upstream | 5′-CGGAATTCTAACAGCTCCTCATTCC-3′ | 169 |
| Downstream | 5′-CGGGATCCATGACAAAGTCTGCTTAGAC-3′ | |||
| Pair 7 | Exon 7-1 | Upstream | 5′-GATGATAGGCACTTTTCTGTGACC-3′ | 239 |
| Downstream | 5′-AGGGCCTTGTGGAAGTGGGGGTCC-3′ | |||
| Pair 8 | Exon 7-2 | Upstream | 5′-CCTCCTCCAGATGAAAGAGATCAC-3′ | 243 |
| Downstream | 5′-CTGTCTATGGGTATCATGTTCATG-3′ | |||
| Pair 9 | Exon 7-3 | Upstream | 5′-CACGGACACCATCCCCATGCACAC-3′ | 213 |
| Downstream | 5′-GCCGTGGCCATGGCAACAGTGACC-3′ | |||
| Pair 10 | Exon 7-4 | Upstream | 5′-CCTTGTGACCCACCACCCCATAAC-3′ | 201 |
| Downstream | 5′-CAATGGGAAGCTGGGAAAATTGGC-3′ | |||
| Pair 11 | Exon 7-5 | Upstream | 5′-AAAAGGTGAGGTGCTGCCACTTCC-3′ | 211 |
| Downstream | 5′-CATTTTCCTCTTCAAAGGAATCAC-3′ | |||
| Pair 12 | Exon 3 | Upstream | 5′-AGTAGGAGGATGTTGCATCAC-3′ | 283 |
| Downstream | 5′-CATAGATGAGACAAATACCAC-3′ |
| Primer . | Region . | Direction . | Sequence . | Length of Product (bp) . |
|---|---|---|---|---|
| Pair 1 | Exon 1 | Upstream | 5′-CGGAATTCTCTGCAGTGGCAGATC-3′ | 256 |
| Downstream | 5′-CGGGATCCTCTGCCATTGGCAATTCC-3′ | |||
| Pair 2 | Exon 2 | Upstream | 5′-CGGAATTCACATGTTGCTTTTGGC-3′ | 184 |
| Downstream | 5′-CGGGATCCAGAGTGAAGGTGCCCTC-3′ | |||
| Pair 3 | Exon 3 | Upstream | 5′-CGGAATTCCTCAAGAGCCTCTTTC-3′ | 149 |
| Downstream | 5′-CGGGATCCAATTTAACAGATTACC-3′ | |||
| Pair 4 | Exon 4 | Upstream | 5′-CGGAATTCACTGCTATCTTCTTTC-3′ | 224 |
| Downstream | 5′-CGGGATCCGAACCTTAGTGGAGAC-3′ | |||
| Pair 5 | Exon 5 | Upstream | 5′-CGGAATTCTTGAAACTATTTGATCCC-3′ | 155 |
| Downstream | 5′-CGGGATCCAACGATCACAGACATTTC-3′ | |||
| Pair 6 | Exon 6 | Upstream | 5′-CGGAATTCTAACAGCTCCTCATTCC-3′ | 169 |
| Downstream | 5′-CGGGATCCATGACAAAGTCTGCTTAGAC-3′ | |||
| Pair 7 | Exon 7-1 | Upstream | 5′-GATGATAGGCACTTTTCTGTGACC-3′ | 239 |
| Downstream | 5′-AGGGCCTTGTGGAAGTGGGGGTCC-3′ | |||
| Pair 8 | Exon 7-2 | Upstream | 5′-CCTCCTCCAGATGAAAGAGATCAC-3′ | 243 |
| Downstream | 5′-CTGTCTATGGGTATCATGTTCATG-3′ | |||
| Pair 9 | Exon 7-3 | Upstream | 5′-CACGGACACCATCCCCATGCACAC-3′ | 213 |
| Downstream | 5′-GCCGTGGCCATGGCAACAGTGACC-3′ | |||
| Pair 10 | Exon 7-4 | Upstream | 5′-CCTTGTGACCCACCACCCCATAAC-3′ | 201 |
| Downstream | 5′-CAATGGGAAGCTGGGAAAATTGGC-3′ | |||
| Pair 11 | Exon 7-5 | Upstream | 5′-AAAAGGTGAGGTGCTGCCACTTCC-3′ | 211 |
| Downstream | 5′-CATTTTCCTCTTCAAAGGAATCAC-3′ | |||
| Pair 12 | Exon 3 | Upstream | 5′-AGTAGGAGGATGTTGCATCAC-3′ | 283 |
| Downstream | 5′-CATAGATGAGACAAATACCAC-3′ |