Quantitation of Cell-Surface TR Expression
| Cell Line . | [125I]ATAP2 Bound . | Surface TR . |
|---|---|---|
| . | (fmol/106 cells) . | (receptors/cell) . |
| HL60 | 0 ± 43 | 0 ± 26,000 |
| MEG-01 | 186 ± 21 | 112,000 ± 12,000 |
| Jurkat | 115 ± 38 | 69,000 ± 23,000 |
| JRT-T3.5 | 113 ± 24 | 68,000 ± 14,000 |
| J.CaM1/rep3 | 171 ± 27 | 103,000 ± 16,000 |
| J.CaM1/lck | 136 ± 34 | 82,000 ± 20,000 |
| J45.01 | 158 ± 29 | 95,000 ± 18,000 |
| J45/CH11 | 148 ± 39 | 89,000 ± 23,000 |
| Cell Line . | [125I]ATAP2 Bound . | Surface TR . |
|---|---|---|
| . | (fmol/106 cells) . | (receptors/cell) . |
| HL60 | 0 ± 43 | 0 ± 26,000 |
| MEG-01 | 186 ± 21 | 112,000 ± 12,000 |
| Jurkat | 115 ± 38 | 69,000 ± 23,000 |
| JRT-T3.5 | 113 ± 24 | 68,000 ± 14,000 |
| J.CaM1/rep3 | 171 ± 27 | 103,000 ± 16,000 |
| J.CaM1/lck | 136 ± 34 | 82,000 ± 20,000 |
| J45.01 | 158 ± 29 | 95,000 ± 18,000 |
| J45/CH11 | 148 ± 39 | 89,000 ± 23,000 |
Direct equilibrium binding of anti-TR MoAb ATAP2 was performed by incubating 106 cells with 125I-labeled ATAP2 for 60 minutes at 4°C. Values represent of mean ± SE of three to five separate determinations.