Densitometric Analysis of c/EBPε and MPO in Human Hematopoietic Cell Lines
| Cell Type . | Cell Line . | C/EBPε/ . | MPO/ . |
|---|---|---|---|
| . | . | β-Actin . | β-Actin . |
| Promyelocyte | NB4 | 2.5 | 1.3 |
| Myeloblast* | GFD8 | 2.26 | 0.16 |
| Late myeloblast | HL-60 | 2.24 | 1.39 |
| Myelomonoblast | THP1 | 0.42 | 0.2 |
| Myeloblast* | MDS92 | 0.33 | 0.87 |
| Early myeloblast | KCL22 | 0.25 | 0 |
| Myelomonoblast | U937 | 0.24 | 0 |
| Nl granulocyte | — | 0.19 | 0.54 |
| Early myeloblast | ML-1 | 0.18 | 1.48 |
| Early myeloblast* | TF-1 | 0.06 | 0 |
| Early myeloblast* | GM150 | 0.05 | 0 |
| Early myeloblast* | AML1 | 0.05 | 1.15 |
| Early myeloblast | KG1 | 0.04 | 0 |
| Nl macrophage | — | 0.04 | 0 |
| Erythroblast | HEL | 0 | 0 |
| Erythroblast | K562 | 0 | 0 |
| Megakaryoblast | MEGA1 | 0 | 0 |
| Basophil precursor | KU812 | 0 | 0 |
| Cell Type . | Cell Line . | C/EBPε/ . | MPO/ . |
|---|---|---|---|
| . | . | β-Actin . | β-Actin . |
| Promyelocyte | NB4 | 2.5 | 1.3 |
| Myeloblast* | GFD8 | 2.26 | 0.16 |
| Late myeloblast | HL-60 | 2.24 | 1.39 |
| Myelomonoblast | THP1 | 0.42 | 0.2 |
| Myeloblast* | MDS92 | 0.33 | 0.87 |
| Early myeloblast | KCL22 | 0.25 | 0 |
| Myelomonoblast | U937 | 0.24 | 0 |
| Nl granulocyte | — | 0.19 | 0.54 |
| Early myeloblast | ML-1 | 0.18 | 1.48 |
| Early myeloblast* | TF-1 | 0.06 | 0 |
| Early myeloblast* | GM150 | 0.05 | 0 |
| Early myeloblast* | AML1 | 0.05 | 1.15 |
| Early myeloblast | KG1 | 0.04 | 0 |
| Nl macrophage | — | 0.04 | 0 |
| Erythroblast | HEL | 0 | 0 |
| Erythroblast | K562 | 0 | 0 |
| Megakaryoblast | MEGA1 | 0 | 0 |
| Basophil precursor | KU812 | 0 | 0 |
Densitometry was performed for all the samples and ratios were calculated between hybridization intensity of β-actin and either C/EBPε or MPO. Most samples were analyzed several times with very similar results.
Abbreviation: Nl, normal.
Growth factor-dependent cell lines.