Table 4. Estimation of Absolute CFU-S,... | American Society of Hematology

Table 4.

Estimation of Absolute CFU-S, CFU-C, and PHSC in Two Femurs and Two Tibias From Either Normal or Mutant Mice

Marrow Type	CFU-GM^4-150	BFU-E	CFU-GEMM	CFU-S	PHSC^4-151
HBD	156,300	11,666	3,313	10,794	399
Normal	53,580	4,497	1,104	5,150	440

Marrow Type	CFU-GM^4-150	BFU-E	CFU-GEMM	CFU-S	PHSC^4-151
HBD	156,300	11,666	3,313	10,794	399
Normal	53,580	4,497	1,104	5,150	440

F4-150

Absolute numbers were calculated by multiplying the CFU concentration (Table 3) by the BM cellularity for two tibias and two femurs. The cellularity was calculated based on the mean of BM isolations for the transplant studies. For hbd/hbd mice (n = 3), marrow cellularity was 7.0 × 10⁷ cells. For +^hbd/+^hbd mice, marrow cellularity was 4.4 × 10⁷.

F4-151

For PHSC numbers, the mean engraftment for white blood cells from both competitive repopulation experiments was determined for the HBD and normal marrows. The fraction of HBD to control was multiplied by the estimated frequency of PHSC in the normal mouse.20 21 Both the estimate for normal and HBD mice was multiplied by their respective cellularities.

For HBD, estimation of absolute PHSC is as follows:

\frac{40.95 (% HBD gpi 1 b)}{72.0 (% normal gpi 1 b) Cells} \times \frac{1 PHSC}{10^{5} Normal BM Cells} = \frac{0.57 PHSC}{10^{5} HBD BM}

\frac{0.57 PHSC}{10^{5} HBD BM Cells} \times 7.0 \times 10^{7} Cells = 399 PHSC

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