Effects of Crotalin on Thromboxane B2Formation of Washed Platelets Stimulated by Thrombin and Other Agonist
| . | Thromboxane B2(ng/mL) . | ||
|---|---|---|---|
| Ristocetin + vWF . | Thrombin . | Collagen . | |
| Control | 12.4 ± 2.6 | 53.3 ± 3.2 | 211.3 ± 12.5 |
| Crotalin | |||
| 5 μg/mL | 3.4 ± 0.6-150 | ND | ND |
| 10 μg/mL | 3.5 ± 0.4-150 | 40.9 ± 3.5 | ND |
| 20 μg/mL | ND | 27.3 ± 3.9-151 | 198.1 ± 10.3 |
| 40 μg/mL | ND | ND | 189.3 ± 9.2 |
| . | Thromboxane B2(ng/mL) . | ||
|---|---|---|---|
| Ristocetin + vWF . | Thrombin . | Collagen . | |
| Control | 12.4 ± 2.6 | 53.3 ± 3.2 | 211.3 ± 12.5 |
| Crotalin | |||
| 5 μg/mL | 3.4 ± 0.6-150 | ND | ND |
| 10 μg/mL | 3.5 ± 0.4-150 | 40.9 ± 3.5 | ND |
| 20 μg/mL | ND | 27.3 ± 3.9-151 | 198.1 ± 10.3 |
| 40 μg/mL | ND | ND | 189.3 ± 9.2 |
Human washed platelets were preincubated with saline or various concentrations of crotalin at 37°C for 5 minutes before the addition of ristocetin (1 mg/mL) plus vWF (10 μg/mL), thrombin (0.03 U/mL), and collagen (10 μg/mL). Six minutes after stimulation, the reaction was terminated by the addition of EDTA (2 mmol/L) and indomethacin (50 μmol/L). The basal value of resting platelets was 2.4 ± 0.5 ng/mL. Values are presented as mean ± SEM.
Abbreviation: ND, not determined.
P < .01 as compared with the respective control, n = 5.
P < .05.