Quantitation of Colony Types in Yolk Sac Hematopoiesis Cultures
| . | CFU-E . | BFU-E . | CFU-GEMM . | CFU-GM . | CFU-G . | CFU-M . | Mean No. Cells per Colony . |
|---|---|---|---|---|---|---|---|
| A. | |||||||
| +/+ | 142.5 ± 138.7 | 34.3 ± 29.7 | 124.3 ± 4.1 | 108.0 ± 34.6 | 12.3 ± 7.5 | 71.8 ± 0.5 | |
| (n = 8) | (121.8 ± 122.8) | (31.8 ± 25.5) | (113.5 ± 83.6) | (99.4 ± 32.7) | (11.6 ± 7.2) | (75.9 ± 49.6) | 1.3 × 104 × 1.1 × 104 (n = 48) |
| +/− | 150.66 ± 94.58 | 25.0 ± 11.1 | 43.0 ± 33.2 | 86.6 ± 37.6 | 13.0 ± 14.7 | 21.0 ± 8.5 | |
| (n = 6) | (168.4 ± 159.2) | (21.0 ± 25.6) | (45.6 ± 41.0) | (82.0 ± 36.8) | (10.4 ± 8.8) | (19.2 ± 5.6) | 1.4 × 104 ± 9.5 × 103 (n = 48) |
| −/− | 112.34 ± 82.1 | 3.0 ± 3.5 | 2.0 ± 2.2 | 53.3 ± 22.54 | 5.7 ± 4.3 | 5.0 ± 4.1 | |
| (n = 6) | (202.0 ± 125.0) | (6.0 ± 5.2) | (2.4 ± 2.6) | (77.0 ± 44.4) | (6.8 ± 4.8) | (5.4 ± 3.4) | 3.2 × 103 ± 2.2 × 103 (n = 48) |
| B. | 51.0 ± 14.06 | 43.0 ± 25.4 | 38.0 ± 14.2 | 71.0 ± 27.6 | 9.0 ± 4.8 | 31.5 ± 17.5 | ND |
| +/− | |||||||
| (n = 4) | (54.2 ± 17.6) | (23.5 ± 17.2) | (20.3 ± 9.5) | (36.5 ± 9.8) | (4.6 ± 2.3) | (17.4 ± 12.3) | |
| −/− | 78.0 ± 17.3 | 8.0 ± 3.5 | 4.7 ± 3.1 | 86.0 ± 61.0 | 2.0 ± 2.0 | 4.7 ± 1.2 | ND |
| (n = 3) | (198.9 ± 25.4) | (10.3 ± 4.3) | (5.7 ± 5.9) | (73.3 ± 52.1) | (4.8 ± 5.0) | (6.2 ± 2.6) |
| . | CFU-E . | BFU-E . | CFU-GEMM . | CFU-GM . | CFU-G . | CFU-M . | Mean No. Cells per Colony . |
|---|---|---|---|---|---|---|---|
| A. | |||||||
| +/+ | 142.5 ± 138.7 | 34.3 ± 29.7 | 124.3 ± 4.1 | 108.0 ± 34.6 | 12.3 ± 7.5 | 71.8 ± 0.5 | |
| (n = 8) | (121.8 ± 122.8) | (31.8 ± 25.5) | (113.5 ± 83.6) | (99.4 ± 32.7) | (11.6 ± 7.2) | (75.9 ± 49.6) | 1.3 × 104 × 1.1 × 104 (n = 48) |
| +/− | 150.66 ± 94.58 | 25.0 ± 11.1 | 43.0 ± 33.2 | 86.6 ± 37.6 | 13.0 ± 14.7 | 21.0 ± 8.5 | |
| (n = 6) | (168.4 ± 159.2) | (21.0 ± 25.6) | (45.6 ± 41.0) | (82.0 ± 36.8) | (10.4 ± 8.8) | (19.2 ± 5.6) | 1.4 × 104 ± 9.5 × 103 (n = 48) |
| −/− | 112.34 ± 82.1 | 3.0 ± 3.5 | 2.0 ± 2.2 | 53.3 ± 22.54 | 5.7 ± 4.3 | 5.0 ± 4.1 | |
| (n = 6) | (202.0 ± 125.0) | (6.0 ± 5.2) | (2.4 ± 2.6) | (77.0 ± 44.4) | (6.8 ± 4.8) | (5.4 ± 3.4) | 3.2 × 103 ± 2.2 × 103 (n = 48) |
| B. | 51.0 ± 14.06 | 43.0 ± 25.4 | 38.0 ± 14.2 | 71.0 ± 27.6 | 9.0 ± 4.8 | 31.5 ± 17.5 | ND |
| +/− | |||||||
| (n = 4) | (54.2 ± 17.6) | (23.5 ± 17.2) | (20.3 ± 9.5) | (36.5 ± 9.8) | (4.6 ± 2.3) | (17.4 ± 12.3) | |
| −/− | 78.0 ± 17.3 | 8.0 ± 3.5 | 4.7 ± 3.1 | 86.0 ± 61.0 | 2.0 ± 2.0 | 4.7 ± 1.2 | ND |
| (n = 3) | (198.9 ± 25.4) | (10.3 ± 4.3) | (5.7 ± 5.9) | (73.3 ± 52.1) | (4.8 ± 5.0) | (6.2 ± 2.6) |
Numbers represent number of colonies of particular morphology per yolk sac. Numbers in parentheses represent numbers of colonies per 105 yolk sac cells plated. CFU-E were scored 3 days after plating in methylcellulose media containing only erythropoietin as an added growth factor. The other colony types were scored after 7 days in culture in methylcellulose media containing IL-3, IL-6, and stem cell factor (A) or IL-3, IL-6, stem cell factor and erythropoietin (B).
Abbreviation: ND, not determined.