Quantitation of Cell Types in Yolk Sac Hematopoiesis Cultures
| . | +/+ . | +/− . | −/− . |
|---|---|---|---|
| Myeloblasts | 1.2 | 2.4 | 4.2 |
| Promyelocytes | 13.0 | 14.0 | 17.4 |
| Myelocytes | 49.2 | 62.4 | 62.2 |
| Metamyelocytes | 5.0 | 4.2 | 5.8 |
| Bands | 2.4 | 1.4 | 1.6 |
| Neutrophils | 6.0 | 1.0 | 1.6 |
| Basophils | 2.0 | 1.2 | 1.4 |
| Monocytes/macrophages | 25.6 | 8.8 | 3.2 |
| Erythroid cells | 1.0 | 4.6 | 2.6 |
| . | +/+ . | +/− . | −/− . |
|---|---|---|---|
| Myeloblasts | 1.2 | 2.4 | 4.2 |
| Promyelocytes | 13.0 | 14.0 | 17.4 |
| Myelocytes | 49.2 | 62.4 | 62.2 |
| Metamyelocytes | 5.0 | 4.2 | 5.8 |
| Bands | 2.4 | 1.4 | 1.6 |
| Neutrophils | 6.0 | 1.0 | 1.6 |
| Basophils | 2.0 | 1.2 | 1.4 |
| Monocytes/macrophages | 25.6 | 8.8 | 3.2 |
| Erythroid cells | 1.0 | 4.6 | 2.6 |
Numbers represent the percentage of each cell type based on a 500 cell differential count on Wright-Giemsa stained smears of yolk sac cultures. Yolk sac cells from day 10.5 embryos were cultured for 10 days in methylcellulose media containing IL-3, IL-6, and stem cell factor.