Absolute Number of Viable DP and CD4+ SP Thymocytes per Well at 36 Hours After Coculture of αβ TCR Transgenic Thymocytes With Thymic DC, Splenic DC, or Splenic B Cells From BALB.D2 Mice at 2:1, 3:1, 5:1, or 10:1, Thymocyte to APC Ratios
| . | + Thymic DC . | + Splenic DC . | + Splenic B cells . | |||
|---|---|---|---|---|---|---|
| . | DP . | CD4 . | DP . | CD4 . | DP . | CD4 . |
| 2:1 | 4.800 | 8.200 | 5.900 | 24.700 | 9.000 | 10.500 |
| 3:1 | 5.800 | 8.600 | 6.600 | 18.000 | 12.900 | 9.200 |
| 5:1 | 5.400 | 7.800 | 7.500 | 11.300 | 15.900 | 7.800 |
| 10:1 | 6.700 | 7.500 | 7.300 | 10.800 | 16.300 | 7.200 |
| . | + Thymic DC . | + Splenic DC . | + Splenic B cells . | |||
|---|---|---|---|---|---|---|
| . | DP . | CD4 . | DP . | CD4 . | DP . | CD4 . |
| 2:1 | 4.800 | 8.200 | 5.900 | 24.700 | 9.000 | 10.500 |
| 3:1 | 5.800 | 8.600 | 6.600 | 18.000 | 12.900 | 9.200 |
| 5:1 | 5.400 | 7.800 | 7.500 | 11.300 | 15.900 | 7.800 |
| 10:1 | 6.700 | 7.500 | 7.300 | 10.800 | 16.300 | 7.200 |
Cultures were set with 2 × 105 thymocytes and varying numbers of APC. Data are representative of three independent experiments with similar results.