The Effect of FcγRIIB1(B1) and FcγRIIB2 (B2) on Phagocytosis by the Chimeric Receptor FcγRIIIAα-γ-γ (EC-TM-CYT)
. | PI . | MFI . | |
---|---|---|---|
α-FcγRIII . | α-FcγRII . | ||
Experiment 1 | |||
α-γ-γ | 140 | 261 | — |
α-γ-γ + B1 | 127 | 227 | 104 |
α-γ-γ + B2 | 132 | 203 | 118 |
Experiment 2 | |||
α-γ-γ | 210 | 169 | — |
α-γ-γ + B1 | 198 | 140 | 102 |
α-γ-γ + B2 | 228 | 130 | 99 |
Experiment 3 | |||
α-γ-γ | 139 | 146 | — |
α-γ-γ + B1 | 103 | 149 | 149 |
α-γ-γ + B2 | 117 | 224 | 179 |
Experiment 4 | |||
α-γ-γ | 76 | 127 | — |
α-γ-γ + B1 | 63 | 128 | 128 |
α-γ-γ + B2 | 66 | 124 | 127 |
. | PI . | MFI . | |
---|---|---|---|
α-FcγRIII . | α-FcγRII . | ||
Experiment 1 | |||
α-γ-γ | 140 | 261 | — |
α-γ-γ + B1 | 127 | 227 | 104 |
α-γ-γ + B2 | 132 | 203 | 118 |
Experiment 2 | |||
α-γ-γ | 210 | 169 | — |
α-γ-γ + B1 | 198 | 140 | 102 |
α-γ-γ + B2 | 228 | 130 | 99 |
Experiment 3 | |||
α-γ-γ | 139 | 146 | — |
α-γ-γ + B1 | 103 | 149 | 149 |
α-γ-γ + B2 | 117 | 224 | 179 |
Experiment 4 | |||
α-γ-γ | 76 | 127 | — |
α-γ-γ + B1 | 63 | 128 | 128 |
α-γ-γ + B2 | 66 | 124 | 127 |
Four representative experiments are shown. The α-γ-γ chimera contains the EC of the FcγRIIIA α chain and the TM and CYT domains of the FcγRIIIA γ chain. B1 and B2 minimally inhibited α-γ-γ phagocytosis. Cell-surface expression of α-γ-γ was similar within each experiment and was not reduced by the expression of FcγRIIB. α-FcγRIII, anti-FcγRIII (recognizes α-γ-γ), α-FcγRII, anti-FcγRII (recognizes FcγRIIB).