In Vitro Expansion of Human Hematopoietic Cells
| Growth Factors . | Days . | |||
|---|---|---|---|---|
| 0 . | 14 . | |||
| TNCC (×103) . | CFU-C . | TNCC (×103) . | CFU-C . | |
| Experiment no. 1 | ||||
| FL, SF, IL-6, EP | 6 | 1,380 | 940 (×157) | 15,040 (×11) |
| FL, SF, IL-6, EP, IL-3 | 6 | 1,380 | 2,400 (×400) | 31,200 (×23) |
| Experiment no. 2 | ||||
| FL, SF, IL-6, EP | 6 | 1,740 | 11.7 (×2.0) | 784 (×0.5) |
| FL, SF, IL-6, EP, IL-3 | 6 | 1,740 | 79.7 (×13) | 3,045 (×1.7) |
| Experiment no. 3 | ||||
| FL, SF, IL-6, EP | 6 | 1,710 | 5,700 (×950) | 17,100 (×10) |
| FL, SF, IL-6, EP, IL-3 | 6 | 1,710 | 5,770 (×962) | 25,390 (×15) |
| Growth Factors . | Days . | |||
|---|---|---|---|---|
| 0 . | 14 . | |||
| TNCC (×103) . | CFU-C . | TNCC (×103) . | CFU-C . | |
| Experiment no. 1 | ||||
| FL, SF, IL-6, EP | 6 | 1,380 | 940 (×157) | 15,040 (×11) |
| FL, SF, IL-6, EP, IL-3 | 6 | 1,380 | 2,400 (×400) | 31,200 (×23) |
| Experiment no. 2 | ||||
| FL, SF, IL-6, EP | 6 | 1,740 | 11.7 (×2.0) | 784 (×0.5) |
| FL, SF, IL-6, EP, IL-3 | 6 | 1,740 | 79.7 (×13) | 3,045 (×1.7) |
| Experiment no. 3 | ||||
| FL, SF, IL-6, EP | 6 | 1,710 | 5,700 (×950) | 17,100 (×10) |
| FL, SF, IL-6, EP, IL-3 | 6 | 1,710 | 5,770 (×962) | 25,390 (×15) |
CD34+/c-kitlow bone marrow cells were cultured in serum-free medium containing growth factors. To analyze CFU-C, 200 (day 0) or 5,000 (day 14) cells were individually plated in 35-mm dishes in the presence of 30% FBS, 1% BSA, 100 U/mL IL-3, 100 ng/mL IL-6, 100 ng/mL SF, and 2 U/mL EP. Dishes were examined on day 14 of culture. The numbers in the brackets denote fold increases over day 0 measurements.