Engraftment of Human Cells in Primary Recipients (Fetuses)
| Experiment No. . | Animal No. . | IL-3 in Culture . | % CD45+ Cells in BM . | Human/Total Colonies (%) . | Chimeric/Total Animals . | |
|---|---|---|---|---|---|---|
| Mix . | GM . | |||||
| [Fresh Cells] | ||||||
| 1 | 304 | 2.82 | 3.1 | 6.2 | 3/3 | |
| 305 | 1.96 | 3.1 | 2.4 | |||
| 306 | 0.42 | 0 | 2.2 | |||
| 2 | 20 | 5.3 | 4.8 | 10.3 | 2/4 | |
| 21 | 3.2 | 3.6 | 12.8 | |||
| 3 | 11 | 0.82 | 2.7 | 4.7 | 3/6 | |
| 14 | 1.2 | 4.2 | 9.3 | |||
| 15 | 0.62 | 4.0 | 3.2 | |||
| [Cultured Cells] | ||||||
| 1 | 401 | − | 7.8 | 2.6 | 16.2 | 5/6 |
| 405 | − | 6.3 | 1.6 | 11.3 | ||
| 407 | + | 2.0 | 0 | 2.8 | ||
| 409 | + | 1.8 | 0.9 | 3.8 | ||
| 411 | + | 2.0 | 0 | 2.7 | ||
| 2 | 32 | + | 5.8 | 0 | 9.6 | 3/4 |
| 33 | + | 6.9 | 3.8 | 8.8 | ||
| 35 | − | 3.7 | 2.3 | 10.9 | ||
| 3 | 42 | + | 0.4 | 2.3 | 5.8 | 7/12 |
| 43 | + | 1.7 | 6.2 | 11.4 | ||
| 44 | + | 2.9 | 0.8 | 4.0 | ||
| 46 | − | 2.3 | 0 | 4.9 | ||
| 47 | − | 0.6 | 1.3 | 4.0 | ||
| 49 | − | 1.1 | 2.6 | 0 | ||
| 50 | − | 4.1 | 3.3 | 7.2 | ||
| Experiment No. . | Animal No. . | IL-3 in Culture . | % CD45+ Cells in BM . | Human/Total Colonies (%) . | Chimeric/Total Animals . | |
|---|---|---|---|---|---|---|
| Mix . | GM . | |||||
| [Fresh Cells] | ||||||
| 1 | 304 | 2.82 | 3.1 | 6.2 | 3/3 | |
| 305 | 1.96 | 3.1 | 2.4 | |||
| 306 | 0.42 | 0 | 2.2 | |||
| 2 | 20 | 5.3 | 4.8 | 10.3 | 2/4 | |
| 21 | 3.2 | 3.6 | 12.8 | |||
| 3 | 11 | 0.82 | 2.7 | 4.7 | 3/6 | |
| 14 | 1.2 | 4.2 | 9.3 | |||
| 15 | 0.62 | 4.0 | 3.2 | |||
| [Cultured Cells] | ||||||
| 1 | 401 | − | 7.8 | 2.6 | 16.2 | 5/6 |
| 405 | − | 6.3 | 1.6 | 11.3 | ||
| 407 | + | 2.0 | 0 | 2.8 | ||
| 409 | + | 1.8 | 0.9 | 3.8 | ||
| 411 | + | 2.0 | 0 | 2.7 | ||
| 2 | 32 | + | 5.8 | 0 | 9.6 | 3/4 |
| 33 | + | 6.9 | 3.8 | 8.8 | ||
| 35 | − | 3.7 | 2.3 | 10.9 | ||
| 3 | 42 | + | 0.4 | 2.3 | 5.8 | 7/12 |
| 43 | + | 1.7 | 6.2 | 11.4 | ||
| 44 | + | 2.9 | 0.8 | 4.0 | ||
| 46 | − | 2.3 | 0 | 4.9 | ||
| 47 | − | 0.6 | 1.3 | 4.0 | ||
| 49 | − | 1.1 | 2.6 | 0 | ||
| 50 | − | 4.1 | 3.3 | 7.2 | ||
Analysis was performed on day-60 posttransplantation fetuses.
Abbreviations: Mix, erythrocyte-containing mixed colonies; GM, granulocyte-macrophage colonies.