Flow Cytometric Analyses of Transfected CHO Cells
| No. . | Antibody . | Subunit/Receptor . | MFI* . | |
|---|---|---|---|---|
| WT . | Patient LW . | |||
| 1 | Tab | GPIIb | 42.6† | 25.8 |
| 10E5 | GPIIb/IIIa | 41.3 | 4.2 | |
| 2 | Tab | GPIIb | 56.8 | 34.6 |
| 7E3 | GPIIb/IIIa + αvβ3 | 50.1 | <1.0 | |
| No. . | Antibody . | Subunit/Receptor . | MFI* . | |
|---|---|---|---|---|
| WT . | Patient LW . | |||
| 1 | Tab | GPIIb | 42.6† | 25.8 |
| 10E5 | GPIIb/IIIa | 41.3 | 4.2 | |
| 2 | Tab | GPIIb | 56.8 | 34.6 |
| 7E3 | GPIIb/IIIa + αvβ3 | 50.1 | <1.0 | |
*Mean fluorescence intensity in arbitrary fluorescence units.
Each value represents the average MFI, minus background values from mock transfected cells, from 2 separate experiments. Values for mock transfected cells: experiment 1 Tab = 54.4 and 10E5 = 52.2, experiment 2 Tab = 120.5 and 7E3 = 115.6. Transfection efficiencies were determined by secreted hGH levels using 125I-anti-hGH antibody (expressed in cpm): experiment 1 WT = 4797 and LW = 5539, experiment 2 WT = 1913 and LW = 2148.