Functional Characterization of EBV-Specific CD8+ T-Cell Clones From Donor MJC
| Clone . | % Kill-150 . | IFNγ-151 . | IL-4-151 . | IL-10-151 . |
|---|---|---|---|---|
| 3bE10 | 0.1 | >5,000 | 145 (120-175) | 156 (150-161) |
| 3F5 | 0.5 | >5,000 | 249 (238-260) | 0 |
| 3E11 | 1.0 | 4,215 (4,150-4,280) | 61 (43-80) | 375 (355-395) |
| 3F9 | 1.4 | 4,812 (4,625-5,000) | >400 | 95 (75-116) |
| 10F10 | 3.0 | >5,000 | >400 | ND |
| 3D7 | 3.1 | >5,000 | >400 | 117 (107-127) |
| 10D1 | 5.2 | >5,000 | 309 (295-331) | 43 (40-46) |
| 3H1 | 11.2 | >5,000 | 195 (186-205) | 46 (7-85) |
| 3B2 | 17.0 | 4,258 | 114 | ND |
| 10H11 | 18.9 | >5,000 | >400 | 104 (69-138) |
| 3G4 | 23.4 | 4,476 (3,970-5,000) | 19 (16-22) | 0 |
| 3A5 | 34.1 | 1,723 (1,580-1,870) | 24 (3-61) | 39 (38-40) |
| 3E7 | 40.9 | 143 (140-145) | 197 (196-198) | 0 |
| 3C12 | 42.2 | 545 (536-554) | 268 (232-307) | 0 |
| 3aB4 | 43.2 | 2,528 (2,523-2,533) | 76 (28-124) | 59 (48-70) |
| 3G7 | 44.9 | 884 (880-890) | 132 (64-203) | 66 (35-97) |
| 3D6 | 45.0 | 2,090 (1,858-2,285) | 35 (14-50) | 0 |
| 3bA3 | 46.5 | 629 (605-654) | 161 (149-171) | 0 |
| 3G1 | 48.2 | 1,420 | 46 (23-69) | 0 |
| 3G2 | 48.2 | 3,047 (2,750-3,380) | 10 (4-16) | 0 |
| 3F2 | 48.6 | 403 (385-420) | 48 (45-52) | 0 |
| 3C1 | 51.4 | 2,555 (2,393-2,748) | 14 | 0 |
| 3D11 | 53.0 | 500 (475-525) | 145 (139-152) | 0 |
| 3F11 | 53.6 | 1,439 (1,335-1,540) | 170 (127-212) | 0 |
| 3E8 | 60.3 | 745 (710-780) | 94 (70-119) | 0 |
| 3aH11 | 62.5 | 829 (827-831) | 53 (19-93) | 0 |
| 3H5 | 64.2 | 498 (439-558) | 32 (31-33) | 0 |
| Clone . | % Kill-150 . | IFNγ-151 . | IL-4-151 . | IL-10-151 . |
|---|---|---|---|---|
| 3bE10 | 0.1 | >5,000 | 145 (120-175) | 156 (150-161) |
| 3F5 | 0.5 | >5,000 | 249 (238-260) | 0 |
| 3E11 | 1.0 | 4,215 (4,150-4,280) | 61 (43-80) | 375 (355-395) |
| 3F9 | 1.4 | 4,812 (4,625-5,000) | >400 | 95 (75-116) |
| 10F10 | 3.0 | >5,000 | >400 | ND |
| 3D7 | 3.1 | >5,000 | >400 | 117 (107-127) |
| 10D1 | 5.2 | >5,000 | 309 (295-331) | 43 (40-46) |
| 3H1 | 11.2 | >5,000 | 195 (186-205) | 46 (7-85) |
| 3B2 | 17.0 | 4,258 | 114 | ND |
| 10H11 | 18.9 | >5,000 | >400 | 104 (69-138) |
| 3G4 | 23.4 | 4,476 (3,970-5,000) | 19 (16-22) | 0 |
| 3A5 | 34.1 | 1,723 (1,580-1,870) | 24 (3-61) | 39 (38-40) |
| 3E7 | 40.9 | 143 (140-145) | 197 (196-198) | 0 |
| 3C12 | 42.2 | 545 (536-554) | 268 (232-307) | 0 |
| 3aB4 | 43.2 | 2,528 (2,523-2,533) | 76 (28-124) | 59 (48-70) |
| 3G7 | 44.9 | 884 (880-890) | 132 (64-203) | 66 (35-97) |
| 3D6 | 45.0 | 2,090 (1,858-2,285) | 35 (14-50) | 0 |
| 3bA3 | 46.5 | 629 (605-654) | 161 (149-171) | 0 |
| 3G1 | 48.2 | 1,420 | 46 (23-69) | 0 |
| 3G2 | 48.2 | 3,047 (2,750-3,380) | 10 (4-16) | 0 |
| 3F2 | 48.6 | 403 (385-420) | 48 (45-52) | 0 |
| 3C1 | 51.4 | 2,555 (2,393-2,748) | 14 | 0 |
| 3D11 | 53.0 | 500 (475-525) | 145 (139-152) | 0 |
| 3F11 | 53.6 | 1,439 (1,335-1,540) | 170 (127-212) | 0 |
| 3E8 | 60.3 | 745 (710-780) | 94 (70-119) | 0 |
| 3aH11 | 62.5 | 829 (827-831) | 53 (19-93) | 0 |
| 3H5 | 64.2 | 498 (439-558) | 32 (31-33) | 0 |
Abbreviation: ND, not done.
Percentage of cytotoxicity against autologous LCL in a 5-hour51Cr-release assay at an effector:target ratio of 4:1.
Cytokine titers are expressed as picograms per milliliter and are the mean of two values or, in the case of IL-4, four values from two independent experiments, measured by ELISA of 48-hour supernatants from anti-CD3–activated T-cell cultures, as described in Materials and Methods. Values listed as greater than exceeded the maximum readable ELISA titer.