Elimination of Primary Tumor Cells Isolated From Patients With Breast Cancer During Culture in the Presence of IT
| Patient No. . | Age (yr) . | Cell Source . | Receptor Expression . | Tumor Cell Depletion . | ||
|---|---|---|---|---|---|---|
| Erb-B2 . | EGFR . | Anti–Erb-B2 IT . | Anti–EGFR-IT . | |||
| 1 | 61 | Ascites | + | + | —* | >99.2% |
| 2 | 48 | Pleural effusion | + | + | >99.2% | >99.2% |
| 3 | 73 | Pleural effusion | − | + | — | >99.2% |
| 4 | 74 | Bone marrow | ND | + | — | >99.2% |
| 5 | 84 | Pleural effusion | + | + | — | 75% |
| 6 | 49 | Ascites | + | − | 66% | ND |
| 7 | 68 | Ascites | + | − | 74% | 73% |
| Patient No. . | Age (yr) . | Cell Source . | Receptor Expression . | Tumor Cell Depletion . | ||
|---|---|---|---|---|---|---|
| Erb-B2 . | EGFR . | Anti–Erb-B2 IT . | Anti–EGFR-IT . | |||
| 1 | 61 | Ascites | + | + | —* | >99.2% |
| 2 | 48 | Pleural effusion | + | + | >99.2% | >99.2% |
| 3 | 73 | Pleural effusion | − | + | — | >99.2% |
| 4 | 74 | Bone marrow | ND | + | — | >99.2% |
| 5 | 84 | Pleural effusion | + | + | — | 75% |
| 6 | 49 | Ascites | + | − | 66% | ND |
| 7 | 68 | Ascites | + | − | 74% | 73% |
Tumor cells (>60% purity) were cultured in a-MEM/10% FCS for 7 days in the presence of 1,000 ng/mL ITs as described in Materials and Methods. Tumor cells were quantitated by immunohistochemical staining with anticytokeratin antibodies.
Abbreviations: EGFR, EGF receptor; +, receptor expression detectable by Western blot; −, receptor expression undetectable by Western blot; ND, not determined.
—, No response detected. Percent values represent the percent depletion of cytokeratin-positive cells in treated cultures compared with untreated control cultures.