Effect of Leptin on Colony Formation From BMC of 5-FU–Treated Mice in Serum-Free Culture
| Factors . | No. of Colonies per 5 × 104 Cells . | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| . | GM . | E . | Mk . | EM . | GMM . | GEM . | GEMM . | Blast . | Total . |
| None | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| Leptin | 3 ± 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 ± 1 |
| Epo | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| Leptin + Epo | 3 ± 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 ± 1 |
| SCF + Epo | 24 ± 7 | 3 ± 1 | 0 | 0 | 0 | 2 ± 1 | 0 | 2 ± 1 | 30 ± 8 |
| IL-6 + Epo | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| Leptin + SCF + Epo | 57 ± 143-150 | 1 ± 2 | 2 ± 3 | 1 ± 1 | 5 ± 3 | 6 ± 7 | 8 ± 5 | 2 ± 2 | 82 ± 193-150 |
| IL-6 + SCF + Epo | 52 ± 19 | 2 ± 1 | 2 ± 2 | 2 ± 1 | 4 ± 3 | 5 ± 3 | 8 ± 5 | 1 ± 1 | 75 ± 19 |
| Leptin + IL-6 + Epo | 7 ± 4 | 0 | 0 | 0 | 4 ± 6 | 0 | 0 | 0 ± 1 | 8 ± 4 |
| Factors . | No. of Colonies per 5 × 104 Cells . | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| . | GM . | E . | Mk . | EM . | GMM . | GEM . | GEMM . | Blast . | Total . |
| None | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| Leptin | 3 ± 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 ± 1 |
| Epo | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| Leptin + Epo | 3 ± 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 ± 1 |
| SCF + Epo | 24 ± 7 | 3 ± 1 | 0 | 0 | 0 | 2 ± 1 | 0 | 2 ± 1 | 30 ± 8 |
| IL-6 + Epo | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| Leptin + SCF + Epo | 57 ± 143-150 | 1 ± 2 | 2 ± 3 | 1 ± 1 | 5 ± 3 | 6 ± 7 | 8 ± 5 | 2 ± 2 | 82 ± 193-150 |
| IL-6 + SCF + Epo | 52 ± 19 | 2 ± 1 | 2 ± 2 | 2 ± 1 | 4 ± 3 | 5 ± 3 | 8 ± 5 | 1 ± 1 | 75 ± 19 |
| Leptin + IL-6 + Epo | 7 ± 4 | 0 | 0 | 0 | 4 ± 6 | 0 | 0 | 0 ± 1 | 8 ± 4 |
The number of colonies indicates mean ± SD of triplicate cultures.
Significantly different from their counterparts without leptin (P < .05).