Replating of Blast Cell Colonies Induced by Leptin and SCF
| Colony No. . | No. of Secondary Colonies per 1/2 Primary Colony . | |||||||
|---|---|---|---|---|---|---|---|---|
| . | GM . | E . | Mk . | EM . | GEM . | GMM . | GEMM . | Total . |
| 1 | 26 | 0 | 0 | 0 | 0 | 2 | 5 | 33 |
| 2 | 25 | 0 | 2 | 0 | 21 | 0 | 3 | 51 |
| 3 | 15 | 1 | 1 | 0 | 0 | 0 | 2 | 19 |
| 4 | 30 | 0 | 0 | 0 | 0 | 0 | 0 | 30 |
| 5 | 40 | 0 | 1 | 0 | 8 | 1 | 1 | 51 |
| 6 | 55 | 0 | 2 | 4 | 25 | 1 | 2 | 89 |
| 7 | 39 | 0 | 0 | 0 | 0 | 0 | 0 | 39 |
| 8 | 45 | 0 | 0 | 1 | 0 | 1 | 0 | 47 |
| Colony No. . | No. of Secondary Colonies per 1/2 Primary Colony . | |||||||
|---|---|---|---|---|---|---|---|---|
| . | GM . | E . | Mk . | EM . | GEM . | GMM . | GEMM . | Total . |
| 1 | 26 | 0 | 0 | 0 | 0 | 2 | 5 | 33 |
| 2 | 25 | 0 | 2 | 0 | 21 | 0 | 3 | 51 |
| 3 | 15 | 1 | 1 | 0 | 0 | 0 | 2 | 19 |
| 4 | 30 | 0 | 0 | 0 | 0 | 0 | 0 | 30 |
| 5 | 40 | 0 | 1 | 0 | 8 | 1 | 1 | 51 |
| 6 | 55 | 0 | 2 | 4 | 25 | 1 | 2 | 89 |
| 7 | 39 | 0 | 0 | 0 | 0 | 0 | 0 | 39 |
| 8 | 45 | 0 | 0 | 1 | 0 | 1 | 0 | 47 |
Each blast cell colony consisting of 50 to 150 cells was resuspended in 0.2 mL of α-medium, and divided into two aliquots; 0.1 mL was replated in secondary culture supplemented with SCF, IL-3, IL-6, G-CSF, Epo, and Tpo, and the remaining 0.1 mL was used for morphologic examination to confirm its blastic character.