Assessment of FISH and CD45 for Analysis of Engraftment of Human Cells in NOD/SCID Mice
| % Human Cells Diluted in Mouse Cells . | FISH (D8Z2) (%)* . | CD45 (%) . | |
|---|---|---|---|
| Scorer 1 . | Scorer 2 . | ||
| 0 | 0 | 0 | 0 |
| 0 | 0 | 0 | 0.1 |
| 0 | 0 | 0 | 0 |
| 1 | 1 | 0.5 | 1.2 |
| 2 | 2 | 3 | 3.3 |
| 3 | 2.7 | 3 | 1.8 |
| 5 | 5 | 4 | 4.6 |
| 10 | 7.3 | 4 | 6.9 |
| 25 | 29 | 27 | 22.3 |
| 50 | 36 | 46 | 49.9 |
| 75 | 70.3 | 72 | 69.4 |
| 100 | 98 | 99 | 88.3 |
| % Human Cells Diluted in Mouse Cells . | FISH (D8Z2) (%)* . | CD45 (%) . | |
|---|---|---|---|
| Scorer 1 . | Scorer 2 . | ||
| 0 | 0 | 0 | 0 |
| 0 | 0 | 0 | 0.1 |
| 0 | 0 | 0 | 0 |
| 1 | 1 | 0.5 | 1.2 |
| 2 | 2 | 3 | 3.3 |
| 3 | 2.7 | 3 | 1.8 |
| 5 | 5 | 4 | 4.6 |
| 10 | 7.3 | 4 | 6.9 |
| 25 | 29 | 27 | 22.3 |
| 50 | 36 | 46 | 49.9 |
| 75 | 70.3 | 72 | 69.4 |
| 100 | 98 | 99 | 88.3 |
A known dilution of human cells into mouse cells was tested.
Three hundred cells counted in a blinded fashion.