IL-12 and IFN-α Production by PBMC During Activation With PHA or PHA + IFN-γ
| Activation Condition . | [IL-12] (pg/mL) . | [IFN-α] (pg/mL) . | ||||
|---|---|---|---|---|---|---|
| 24 h . | 48 h . | 72 h . | 24 h . | 48 h . | 72 h . | |
| PHA | ND | ND | ND | ND | ND | ND |
| PHA + IFN-γ | 8 ± 4 | 26 ± 15 | 8 ± 6 | ND | ND | ND |
| Activation Condition . | [IL-12] (pg/mL) . | [IFN-α] (pg/mL) . | ||||
|---|---|---|---|---|---|---|
| 24 h . | 48 h . | 72 h . | 24 h . | 48 h . | 72 h . | |
| PHA | ND | ND | ND | ND | ND | ND |
| PHA + IFN-γ | 8 ± 4 | 26 ± 15 | 8 ± 6 | ND | ND | ND |
PBMC were cultured at 1 × 106 cells/mL in medium containing PHA alone or PHA + IFN-γ. Aliquots of culture supernatants were harvested at the indicated time points and IL-12 and IFN-α concentration was measured using an IL-12 ELISA (detecting only the p70 IL-12 heterodimer) or IFN-α ELISA. Similar results were obtained in three independent experiments.
Abbreviations: ND, not detectable by IL-12 or IFN-α ELISA (detection limit, <3 pg/mL).