The IFN-γ–Induced Upregulation of High-Affinity IL-12 Receptor Expression During T-Cell Activation Is Inhibited by Neutralizing Endogenous IL-12
| Activation Condition . | High-Affinity IL-12R . | Low-Affinity IL-12R . | ||
|---|---|---|---|---|
| kd* (pmol/L) . | Sites/Cell . | kd (nmol/L) . | Sites/Cell . | |
| PHA | 61 ± 20 | 259 ± 59 | 6.0 ± 2.4 | 2,775 ± 318 |
| PHA + IFN-γ | 115 ± 21 | 470 ± 75 | 5.4 ± 1.5 | 2,925 ± 530 |
| PHA + IFN-γ + anti–IL-12 | 88 ± 18 | 325 ± 49 | 5.5 ± 1.4 | 3,325 ± 35 |
| Activation Condition . | High-Affinity IL-12R . | Low-Affinity IL-12R . | ||
|---|---|---|---|---|
| kd* (pmol/L) . | Sites/Cell . | kd (nmol/L) . | Sites/Cell . | |
| PHA | 61 ± 20 | 259 ± 59 | 6.0 ± 2.4 | 2,775 ± 318 |
| PHA + IFN-γ | 115 ± 21 | 470 ± 75 | 5.4 ± 1.5 | 2,925 ± 530 |
| PHA + IFN-γ + anti–IL-12 | 88 ± 18 | 325 ± 49 | 5.5 ± 1.4 | 3,325 ± 35 |
*Mean values and standard deviations for kd and sites/cell are based on data obtained from three separate experiments. Each experiment consisted of radiolabeled IL-12 binding assays performed on T cells from a single donor (using a different donor for each experiment) that were activated under the indicated conditions.