Table 1. Binding Constants for the... | American Society of Hematology

Table 1.

Binding Constants for the Association of t-PA With Fibrin in the Absence and Presence of Ultrasound

	Ultrasound	Kd₁^-150	Kd₂^-150	Bmax_max^-151	Molar Binding Ratio^-152
Fibrin monomer	No	31 nmol/L (1)	244 nmol/L (8)	67 μmol/L (0.5)	3.8 ± .03
	Yes	46 nmol/L (2)	243 nmol/L (8)	69 μmol/L (0.6)	3.8 ± .03
	P < .0001		P = NS	P = NS
Non-crosslinked fibrin	No	267 nmol/L (17)	952 nmol/L (67)	31 μmol/L (0.6)	1.77 ± .04
	Yes	232 nmol/L (10)	917 nmol/L (47)	43 μmol/L (1)	2.42 ± .06
		P = NS	P = NS	P < .0001
Crosslinked fibrin	No	405 nmol/L (23)	—	22 μmol/L (1)	1.27 ± .06
	Yes	343 nmol/L (14)	—	25 μmol/L (0.4)	1.44 ± .02
		P = .026		P < .015

	Ultrasound	Kd₁^-150	Kd₂^-150	Bmax_max^-151	Molar Binding Ratio^-152
Fibrin monomer	No	31 nmol/L (1)	244 nmol/L (8)	67 μmol/L (0.5)	3.8 ± .03
	Yes	46 nmol/L (2)	243 nmol/L (8)	69 μmol/L (0.6)	3.8 ± .03
	P < .0001		P = NS	P = NS
Non-crosslinked fibrin	No	267 nmol/L (17)	952 nmol/L (67)	31 μmol/L (0.6)	1.77 ± .04
	Yes	232 nmol/L (10)	917 nmol/L (47)	43 μmol/L (1)	2.42 ± .06
		P = NS	P = NS	P < .0001
Crosslinked fibrin	No	405 nmol/L (23)	—	22 μmol/L (1)	1.27 ± .06
	Yes	343 nmol/L (14)	—	25 μmol/L (0.4)	1.44 ± .02
		P = .026		P < .015

Numbers in parentheses are 1 SD.

Determined by Scatchard analysis.

Determined from nonlinear regression.

Expressed as moles of t-PA per mole of fibrin. Determined at maximum t-PA binding.