Table 2.

Effect of Hematocrit Level on the PL50 and NA50

Compounds n IC50 (μmol/L)PC50 (μmol/L) PE50 (μmol/L)NA50 (μmol/L) Hematocrit Level Effect
B4 C12H25-NH-CH(C2H5)-CH2OH  12  0.61  7.2 5 7.5 Yes 
    67  36 44  
E4 C10H21-N+(CH3)3 10  0.7  5 80 145 No  
    9.5  115  200  
E6 C12H25-N+(CH3)3 12  0.5  3.2 8 8.5 Yes  
    12  25  300  
E7 C14H29-N+(CH3)3 14  0.9  1.8 2.9 4.6 Yes  
    18  16  18  
E40 C14H29-H+(CH3)2-CH2Φ  14  1  5.5 4.4 2 Yes  
    22  27 19  
E8 C16H33-N+(CH3)3 16  0.8  2.6 2.2 6.6 Yes  
    21  23 44  
F3 C10H21-N+(CH3)2-(CH2)2OH  10  0.97 7 33 62 No 
    18  78  90  
F4 C12H25-N+(CH3)2-(CH2)2OH  12  0.48 2.2 12 8 No 
    6.5  28  19  
F5 C14H29-N+(CH3)2-(CH2)2OH  14  0.6 5.5 5.1 11.5 Yes 
    47  43  75  
F9 C14H29-N+(C2H5)2-(CH2)2OH  14  0.55 3.7 5.7 6.8 Yes 
    26  >30  >30  
F6 C18H37-N+(CH3)3 18  1.3  6 7.5 5.2 Yes  
    24  48 28  
G3 (CH3)3N+-C10H21-N+(CH3)3 10  1.7 12 >200 >200 No 
    16  >200  >200  
G4 (CH3)3N+-C12H25-N+(CH3)3 12 0.09  4.8 >20 >20 No  
    6  >20  >20   
Compounds n IC50 (μmol/L)PC50 (μmol/L) PE50 (μmol/L)NA50 (μmol/L) Hematocrit Level Effect
B4 C12H25-NH-CH(C2H5)-CH2OH  12  0.61  7.2 5 7.5 Yes 
    67  36 44  
E4 C10H21-N+(CH3)3 10  0.7  5 80 145 No  
    9.5  115  200  
E6 C12H25-N+(CH3)3 12  0.5  3.2 8 8.5 Yes  
    12  25  300  
E7 C14H29-N+(CH3)3 14  0.9  1.8 2.9 4.6 Yes  
    18  16  18  
E40 C14H29-H+(CH3)2-CH2Φ  14  1  5.5 4.4 2 Yes  
    22  27 19  
E8 C16H33-N+(CH3)3 16  0.8  2.6 2.2 6.6 Yes  
    21  23 44  
F3 C10H21-N+(CH3)2-(CH2)2OH  10  0.97 7 33 62 No 
    18  78  90  
F4 C12H25-N+(CH3)2-(CH2)2OH  12  0.48 2.2 12 8 No 
    6.5  28  19  
F5 C14H29-N+(CH3)2-(CH2)2OH  14  0.6 5.5 5.1 11.5 Yes 
    47  43  75  
F9 C14H29-N+(C2H5)2-(CH2)2OH  14  0.55 3.7 5.7 6.8 Yes 
    26  >30  >30  
F6 C18H37-N+(CH3)3 18  1.3  6 7.5 5.2 Yes  
    24  48 28  
G3 (CH3)3N+-C10H21-N+(CH3)3 10  1.7 12 >200 >200 No 
    16  >200  >200  
G4 (CH3)3N+-C12H25-N+(CH3)3 12 0.09  4.8 >20 >20 No  
    6  >20  >20   

The PL50 and NA50 were measured and expressed as in Table 1. Experiments were performed as described in Fig2, ie, either at low hematocrit level (2.5 × 106infected cells/300 μL final volume) or at a 10-fold higher hematocrit level (5 × 106 infected cells/60 μL final volume). Values in italics correspond to measurements performed at the lowest hematocrit level (usually between 1.1% and 3%). Yes indicates an effect of hematocrit level on metabolic activity inhibition and no is the opposite; n corresponds to the number of carbons present in the long alkyl chain.

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