IL-6 and IL-10 Production in Lymphoma Cells
| Cell Lines . | KSHV . | EBV . | IL-6 (pg/mL)* . | IL-10 (pg/mL)* . |
|---|---|---|---|---|
| KS-1 | + | − | 720 ± 25 | 370 ± 25 |
| KS-1 + TPA | 250 ± 10 | |||
| KS-1 + IL-6S | 200 ± 20† | |||
| KS-1 + IL-6AS | 50 ± 15† | |||
| KS-1 + IL-10S | 170 ± 50† | |||
| KS-1 + IL-10AS | 10 ± 10† | |||
| BC-1 | + | + | 5,000 ± 500 | 210 ± 30 |
| BC-1 + TPA | 3,000 ± 500 | |||
| BC-1 + IL-6S | 5,000 ± 750† | |||
| BC-1 + IL-6AS | 850 ± 60† | |||
| BC-1 + IL-10S | 150 ± 65† | |||
| BC-1 + IL-10AS | 10 ± 10† | |||
| BC-2 | + | + | 700 ± 60 | 17,000 ± 800 |
| EW36 | − | − | 14 ± 10 | |
| DS179 | − | − | 18 ± 10 |
| Cell Lines . | KSHV . | EBV . | IL-6 (pg/mL)* . | IL-10 (pg/mL)* . |
|---|---|---|---|---|
| KS-1 | + | − | 720 ± 25 | 370 ± 25 |
| KS-1 + TPA | 250 ± 10 | |||
| KS-1 + IL-6S | 200 ± 20† | |||
| KS-1 + IL-6AS | 50 ± 15† | |||
| KS-1 + IL-10S | 170 ± 50† | |||
| KS-1 + IL-10AS | 10 ± 10† | |||
| BC-1 | + | + | 5,000 ± 500 | 210 ± 30 |
| BC-1 + TPA | 3,000 ± 500 | |||
| BC-1 + IL-6S | 5,000 ± 750† | |||
| BC-1 + IL-6AS | 850 ± 60† | |||
| BC-1 + IL-10S | 150 ± 65† | |||
| BC-1 + IL-10AS | 10 ± 10† | |||
| BC-2 | + | + | 700 ± 60 | 17,000 ± 800 |
| EW36 | − | − | 14 ± 10 | |
| DS179 | − | − | 18 ± 10 |
*Results represent the mean (± SD) of three independent experiments with 1 × 106 cells/mL cultured for 48 hours; the IL-6 and IL-10 were measured in the conditioned media.
A total of 1 × 105 cells/mL were cultured with sense or antisense oligonucleotides (10 μmol/L) for 72 hours, and the supernatants were used for IL-6 and IL-10 ELISA assays. IL-6S, IL-6 sense oligonucleotides; IL-6AS, IL-6 antisense oligonucleotides; IL-10S, IL-10 sense oligonucleotides; IL-10 antisense oligonucleotides.