Table 1. Alignment of the Chromodomains... | American Society of Hematology

Table 1.

Alignment of the Chromodomains of thePc Genes and Generation of a Consensus Sequence to Design the Degenerate Oligonucleotides Used in These Studies

Pc Genes	Chromodomain Conserved Sequences
HP1(D)	YVVEKVLDRRVRKGKVEYYLKWKGYPETENTWEPENN^-150
HP1^Hsα(H)	YAVEKIIDRRVVKGQVEYLLKWKGFSEEHNTWEPEKN
HP1^HSγ(H)	FVVEKVLDRRVVNGKVEYFLKWKGFTDADNTWEPEEN
M31(M)	YVVEKVLDRRVVKGKVEYLLKWKGFSDEDNTWEPEEN
M32(M)	FVVEKVLDRRVVNGKVEYFLKWKGFTDADNTWEPEEN
p25(H)^-151	YVVEKYLDRRVVKGKVEYLLKWKGFSDEDNTWEPEEN
Consensus	YVVEKVLDRRVVKGKVEYYLKWKGFPETDNTWEPENN
	FA II RN Q F YSDEH K
	L T AE E

Pc Genes	Chromodomain Conserved Sequences
HP1(D)	YVVEKVLDRRVRKGKVEYYLKWKGYPETENTWEPENN^-150
HP1^Hsα(H)	YAVEKIIDRRVVKGQVEYLLKWKGFSEEHNTWEPEKN
HP1^HSγ(H)	FVVEKVLDRRVVNGKVEYFLKWKGFTDADNTWEPEEN
M31(M)	YVVEKVLDRRVVKGKVEYLLKWKGFSDEDNTWEPEEN
M32(M)	FVVEKVLDRRVVNGKVEYFLKWKGFTDADNTWEPEEN
p25(H)^-151	YVVEKYLDRRVVKGKVEYLLKWKGFSDEDNTWEPEEN
Consensus	YVVEKVLDRRVVKGKVEYYLKWKGFPETDNTWEPENN
	FA II RN Q F YSDEH K
	L T AE E

Abbreviation: D, Drosophila; H, human; M, mouse.

Letters in bold represent the amino acid sequence used to construct the 2 degenerate primers used in these studies.

Human heterochromatin p25 protein is also referred to asHSM1 orHP1^Hsβ and is the human homologue of the mouse M31 gene.