Table 2. Pc Gene Sequences Obtained From... | American Society of Hematology

Table 2.

Pc Gene Sequences Obtained From cDNA Isolated From Purified CD34⁺ Human Bone Marrow Cells With Degenerate Oligonucleotides Designed Based on Conserved Sequences ofPcGenes

Pc Genes	cDNA Sequences Between Degenerate Oligos (and Translation)																					No. of Clones^* Sequenced
		R	V	V	K	G	Q	V	E	Y	L	L	K	W	K	G	F	S	E	E	H
1. HP1^Hsα	G	CGC	GTG	GTT	AAG	GGA	CAA	GTG	GAA	TAT	CTA	CTG	AAG	TGG	AAA	GGC	TTT	TCT	GAG	GAG	CAC	31^†
		H
2. HP1^Hsα-like A	.	.AT	...	...	...	..G	...	...	..G	...	..G	...	...	...	...	...	...	...	...	...	...	3
													E
3. HP1^Hsα-like B	.	...	...	...	...	...	...	...	...	...	...	...	G..	...	...	...	...	...	...	...	...	4^†
																		F
4. HP1^Hsα-like C	.	...	...	...	...	...	...	...	...	...	...	...	...	...	...	...	...	.T.	...	...	...	3^†
		R	V	V	N	G	K	V	E	Y	F	L	K	W	K	G	F	T	D	A	D
5. HP1^Hsγ	A	CGT	GTA	GTG	AAT	GGG	AAA	GTG	GAA	TAT	TTC	CTG	AAG	TGG	AAG	GGA	TTT	ACA	GAT	GCT	GAC	15^†
		R	V	V	K	G	K	V	E	Y	L	L	K	W	K	G	F	S	D	E	D
6. p25	T	CGA	GTG	GTA	AAG	GGC	AAA	GTG	GAG	TAC	CTC	CTA	AAG	TGG	AAG	GGA	TTC	TCA	GAT	GAG	GAC	3
						D										E
7. p25-like	.	...	...	...	...	.A.	...	...	...	...	...	...	...	...	...	.A.	...	...	...	...	..T	1
		R	V	V	N	G	K	V	E	Y	F	L	K	W	K	G	F	T	D	A	D
8. M32 (homologue)	T	CAT	GTA	GTG	AAT	GGG	AAG	GTG	GAG	TAT	TTC	CTG	AAG	TGG	AAG	GGG	TTC	ACA	GAT	GCT	GAT	2^†
9. Several HP1^Hsα-like with a single mutation (considered as potential PCR-induced mutations)																						6
10. One HP1^Hsγ-like with a single mutation																						1

Pc Genes	cDNA Sequences Between Degenerate Oligos (and Translation)																					No. of Clones^* Sequenced
		R	V	V	K	G	Q	V	E	Y	L	L	K	W	K	G	F	S	E	E	H
1. HP1^Hsα	G	CGC	GTG	GTT	AAG	GGA	CAA	GTG	GAA	TAT	CTA	CTG	AAG	TGG	AAA	GGC	TTT	TCT	GAG	GAG	CAC	31^†
		H
2. HP1^Hsα-like A	.	.AT	...	...	...	..G	...	...	..G	...	..G	...	...	...	...	...	...	...	...	...	...	3
													E
3. HP1^Hsα-like B	.	...	...	...	...	...	...	...	...	...	...	...	G..	...	...	...	...	...	...	...	...	4^†
																		F
4. HP1^Hsα-like C	.	...	...	...	...	...	...	...	...	...	...	...	...	...	...	...	...	.T.	...	...	...	3^†
		R	V	V	N	G	K	V	E	Y	F	L	K	W	K	G	F	T	D	A	D
5. HP1^Hsγ	A	CGT	GTA	GTG	AAT	GGG	AAA	GTG	GAA	TAT	TTC	CTG	AAG	TGG	AAG	GGA	TTT	ACA	GAT	GCT	GAC	15^†
		R	V	V	K	G	K	V	E	Y	L	L	K	W	K	G	F	S	D	E	D
6. p25	T	CGA	GTG	GTA	AAG	GGC	AAA	GTG	GAG	TAC	CTC	CTA	AAG	TGG	AAG	GGA	TTC	TCA	GAT	GAG	GAC	3
						D										E
7. p25-like	.	...	...	...	...	.A.	...	...	...	...	...	...	...	...	...	.A.	...	...	...	...	..T	1
		R	V	V	N	G	K	V	E	Y	F	L	K	W	K	G	F	T	D	A	D
8. M32 (homologue)	T	CAT	GTA	GTG	AAT	GGG	AAG	GTG	GAG	TAT	TTC	CTG	AAG	TGG	AAG	GGG	TTC	ACA	GAT	GCT	GAT	2^†
9. Several HP1^Hsα-like with a single mutation (considered as potential PCR-induced mutations)																						6
10. One HP1^Hsγ-like with a single mutation																						1

*Four additional clones were unrelated to Pc-G genes.

†

Sequences obtained from 2 independent PCR reactions.

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