Comparison of Fold Increase of Total Cells and CD34+ Cells in 6-Day Cultures in Single Cytokines or in Cytokine Combinations
| Cytokines . | Fold Increase of Total Cells . | Fold Increase of CD34+ Cells . |
|---|---|---|
| TPO | 0.30 | 0.28 |
| FL | 0.72 ± 0.10 | 0.55 ± 0.06 |
| KL | 0.67 ± 0.15 | 0.56 ± 0.09 |
| TPO, KL* | 1.69 ± 0.47 | 1.02 ± 0.12 |
| TPO, FL | 1.67 ± 0.60 | 1.41 ± 0.50 |
| KL, FL | 1.67 ± 0.14 | 1.08 ± 0.09 |
| TPO, KL, FL* | 4.71 ± 1.50 | 3.43 ± 1.07 |
| IL-3, TPO, FL | 3.17 ± 1.10 | 2.28 ± 0.75 |
| IL-3, IL-6, LIF* | 0.86 ± 0.10 | 0.68 ± 0.08 |
| Cytokines . | Fold Increase of Total Cells . | Fold Increase of CD34+ Cells . |
|---|---|---|
| TPO | 0.30 | 0.28 |
| FL | 0.72 ± 0.10 | 0.55 ± 0.06 |
| KL | 0.67 ± 0.15 | 0.56 ± 0.09 |
| TPO, KL* | 1.69 ± 0.47 | 1.02 ± 0.12 |
| TPO, FL | 1.67 ± 0.60 | 1.41 ± 0.50 |
| KL, FL | 1.67 ± 0.14 | 1.08 ± 0.09 |
| TPO, KL, FL* | 4.71 ± 1.50 | 3.43 ± 1.07 |
| IL-3, TPO, FL | 3.17 ± 1.10 | 2.28 ± 0.75 |
| IL-3, IL-6, LIF* | 0.86 ± 0.10 | 0.68 ± 0.08 |
*Divided and undivided CD34 subpopulations from these cultures were analyzed in the CAFC assay. The values for TPO, KL, FL represent the means ± SEM of six experiments. All other conditions (except TPO [1] and KL [2]) are means ± SEM of three experiments.