Mean CAFC Frequencies of CD34/PKH26 Cell Subsets From 6-Day Cultures
| Cytokines . | Cell Population . | Day of Culture . | CAFC Frequency . |
|---|---|---|---|
| IL-3, IL-6, LIF | CD34+Thy-1+ | 0 | 1/21 (1/16-1/26) |
| CD34hiPKHhi | 6 | 1/21 (1/15-1/23) | |
| CD34hiPKHlo | 6 | 1/440 (1/249-1/813) | |
| TPO, KL | CD34+Thy-1+ | 0 | 1/33 (1/28-1/46) |
| CD34hiPKHhi | 6 | 1/44 (1/37-1/56) | |
| CD34hiPKHlo | 6 | 1/75 (1/59-1/89) | |
| TPO, KL, FL | CD34+Thy-1+ | 0 | 1/46 (1/41-1/52) |
| CD34hiPKHhi * | 6 | ||
| CD34hiPKHlo | 6 | 1/42 (1/33-1/59) | |
| CD34lo/−PKHlo | 6 | 1/2,898 (1/1,743-1/13,415) |
| Cytokines . | Cell Population . | Day of Culture . | CAFC Frequency . |
|---|---|---|---|
| IL-3, IL-6, LIF | CD34+Thy-1+ | 0 | 1/21 (1/16-1/26) |
| CD34hiPKHhi | 6 | 1/21 (1/15-1/23) | |
| CD34hiPKHlo | 6 | 1/440 (1/249-1/813) | |
| TPO, KL | CD34+Thy-1+ | 0 | 1/33 (1/28-1/46) |
| CD34hiPKHhi | 6 | 1/44 (1/37-1/56) | |
| CD34hiPKHlo | 6 | 1/75 (1/59-1/89) | |
| TPO, KL, FL | CD34+Thy-1+ | 0 | 1/46 (1/41-1/52) |
| CD34hiPKHhi * | 6 | ||
| CD34hiPKHlo | 6 | 1/42 (1/33-1/59) | |
| CD34lo/−PKHlo | 6 | 1/2,898 (1/1,743-1/13,415) |
CAFC frequencies at 5 weeks were calculated using maximum likelihood estimation with SAS software.40 The 95% confidence limits are shown in parentheses. Values are the mean of six experiments for TPO, KL, FL and the mean of two experiments for the other cytokine combinations.
Not determined because all cells had undergone division after 6 days of culture in TPO, KL, FL.