The Ability to Give Rise to B-Lymphoid and CD34+ Progenitor Cells in Long-Term Stromal Cultures Was Preserved for CD34hi Cells That Had Divided in TPO, KL, and FL
| Cytokines . | Cell Population . | Day of Culture . | Percentage of Positive Wells . | |
|---|---|---|---|---|
| CD19+ B-lymphoid . | CD34+ Progenitors . | |||
| IL-3, IL-6, LIF | CD34+Thy-1+ | 0 | 71.8 ± 8.2 | 63.2 ± 26.8 |
| CD34hiPKHhi | 6 | 35.9 ± 2.6 | 40.4 ± 9.6 | |
| CD34hiPKHlo | 6 | 0 | 0 | |
| TPO, KL | CD34+Thy-1+ | 0 | 58.2 ± 19.7 | 79.1 ± 9.9 |
| CD34hiPKHhi | 6 | 71.8 ± 5.1 | 28.2 ± 5.1 | |
| CD34hiPKHlo | 6 | 63.4 ± 0.9 | 3.6 ± 3.6 | |
| TPO, KL, FL | CD34+Thy-1+ | 0 | 41.5 ± 16.5 | 66.9 ± 16.8 |
| CD34hiPKHlo | 6 | 54.2 ± 8.5 | 49.0 ± 27.1 | |
| CD34lo/−PKHlo | 6 | ND | ND | |
| Cytokines . | Cell Population . | Day of Culture . | Percentage of Positive Wells . | |
|---|---|---|---|---|
| CD19+ B-lymphoid . | CD34+ Progenitors . | |||
| IL-3, IL-6, LIF | CD34+Thy-1+ | 0 | 71.8 ± 8.2 | 63.2 ± 26.8 |
| CD34hiPKHhi | 6 | 35.9 ± 2.6 | 40.4 ± 9.6 | |
| CD34hiPKHlo | 6 | 0 | 0 | |
| TPO, KL | CD34+Thy-1+ | 0 | 58.2 ± 19.7 | 79.1 ± 9.9 |
| CD34hiPKHhi | 6 | 71.8 ± 5.1 | 28.2 ± 5.1 | |
| CD34hiPKHlo | 6 | 63.4 ± 0.9 | 3.6 ± 3.6 | |
| TPO, KL, FL | CD34+Thy-1+ | 0 | 41.5 ± 16.5 | 66.9 ± 16.8 |
| CD34hiPKHlo | 6 | 54.2 ± 8.5 | 49.0 ± 27.1 | |
| CD34lo/−PKHlo | 6 | ND | ND | |
Wells were scored positive if greater than 1% of cells were positive for the surface marker. All cobblestone areas analyzed contained CD33+ myeloid cells. Values are the means ± SEM for two experiments (IL-3, IL-6, LIF and TPO, KL) or six experiments (TPO, KL, FL).
Abbreviation: ND, not determined due to no or a limited number of wells containing cobblestone areas.