CD34+ Cells That Have Divided During 6 Days of Culture in TPO, KL, and FL Retain Their Capacity for Marrow Repopulation In Vivo in the SCID-hu Bone Assay
| Cell Population . | Experiment . | CAFC Frequency . | Positive Grafts . | % Donor . | % CD19+ . | % CD33+ . | % CD34+ . |
|---|---|---|---|---|---|---|---|
| CD34+Thy-1+ | A | 1/106 | 4/4 | 25.0 ± 13.5 | 22.0 ± 12.5 | 4.8 ± 2.8 | 4.5 ± 0.5 |
| B | 1/38 | ND3-150 | ND | ND | ND | ND | |
| CD34hiPKHlo | A | 1/36 | 4/4 | 34.3 ± 22.3 | 31.3 ± 19.8 | 6.7 ± 6.2 | 3.9 ± 3.4 |
| B | 1/26 | 4/4 | 59.0 ± 12.0 | 58.0 ± 11.5 | 1.9 ± 0.6 | 5.0 ± 1.0 | |
| CD34lo/−PKHlo | A | <1/6,6003-151 | 1/4 | 3.5 ± 5.3 | 3.3 ± 4.9 | 1.3 ± 1.8 | ND |
| B | 1/932 | 0/4 | 0 | 0 | 0 | ND |
| Cell Population . | Experiment . | CAFC Frequency . | Positive Grafts . | % Donor . | % CD19+ . | % CD33+ . | % CD34+ . |
|---|---|---|---|---|---|---|---|
| CD34+Thy-1+ | A | 1/106 | 4/4 | 25.0 ± 13.5 | 22.0 ± 12.5 | 4.8 ± 2.8 | 4.5 ± 0.5 |
| B | 1/38 | ND3-150 | ND | ND | ND | ND | |
| CD34hiPKHlo | A | 1/36 | 4/4 | 34.3 ± 22.3 | 31.3 ± 19.8 | 6.7 ± 6.2 | 3.9 ± 3.4 |
| B | 1/26 | 4/4 | 59.0 ± 12.0 | 58.0 ± 11.5 | 1.9 ± 0.6 | 5.0 ± 1.0 | |
| CD34lo/−PKHlo | A | <1/6,6003-151 | 1/4 | 3.5 ± 5.3 | 3.3 ± 4.9 | 1.3 ± 1.8 | ND |
| B | 1/932 | 0/4 | 0 | 0 | 0 | ND |
Ten thousand cells were injected per bone graft. Errors shown are SEM. Grafts were analyzed 8 weeks after injection for donor cells expressing the HLA marker of the injected cells.
Abbreviation: ND, not determined.
Mice used for injection of uncultured CD34+Thy-1+Lin− cells in experiment B died before analysis of the grafts.
No CAFC detected among 6,600 cells plated.