Effect of Neutralization of IL-18 or IFNγ on LPS-Induced Spleen Cell Proliferation
| . | LPS Alone . | Anti–IL-18 1:400 . | Anti–IL-18 1:200 . | Anti–IL-18 1:100 . | Anti-IFNγ (10 μg/mL) . | Anti-IFNγ (30 μg/mL) . |
|---|---|---|---|---|---|---|
| 48 h | 163.6 ± 7.4 | 176.0 ± 16.2 | 173.9 ± 12.8 | 190.0 ± 13.6* | 179.8 ± 11.6† | 180.3 ± 11.3† |
| 72 h | 164.6 ± 3.8 | 185.8 ± 11.0 | 202.9 ± 19.5† | 207.3 ± 19.7† | 172.4 ± 5.8 | 168.2 ± 5.8 |
| . | LPS Alone . | Anti–IL-18 1:400 . | Anti–IL-18 1:200 . | Anti–IL-18 1:100 . | Anti-IFNγ (10 μg/mL) . | Anti-IFNγ (30 μg/mL) . |
|---|---|---|---|---|---|---|
| 48 h | 163.6 ± 7.4 | 176.0 ± 16.2 | 173.9 ± 12.8 | 190.0 ± 13.6* | 179.8 ± 11.6† | 180.3 ± 11.3† |
| 72 h | 164.6 ± 3.8 | 185.8 ± 11.0 | 202.9 ± 19.5† | 207.3 ± 19.7† | 172.4 ± 5.8 | 168.2 ± 5.8 |
Data are expressed as the percentage of change in MTS absorbance compared with unstimulated cells (100%). Splenocytes from WT mice were incubated with LPS (20 μg/mL) for 48 or 72 hours in the presence or absence of rabbit antimurine IL-18 antiserum at the indicated dilutions or rat antimouse IFNγ at the indicated concentrations. Addition to the cultures of normal rabbit serum at the same concentrations did not significantly alter cell proliferation (data not shown). Data are the mean ± SEM of 3 mice per group.
P < .05.
P < .01 versus respective LPS alone by ANOVA for repeated measures.